Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 18P
The transcription factor Pax6 is required continually during the life of a mouse (or a human) for the development and maintenance of the retina. Homozygous Pax6 knockout mice die soon after birth because Pax6 protein is also required in essential organs, such as the pancreas.
| a. | In order to study the role of Pax6 in eye development, a researcher wants to generate a mouse that expresses Pax6 everywhere except in its eyes. Describe how you could construct such a mouse. |
| b. | Suppose the scientist wants to create a mouse similar to that in part (a), but in which the eye cells from which Pax6 function has been removed now express GFP. Marking the cells in this way will allow the investigator to see the shapes of the Pax6 eye cells more easily than if they did not express GFP. Diagram a Pax6 gene construct that would enable her to do this experiment. |
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The transcription factor Pax6 is required continually during the life of a mouse (or a human)
for the development of the retina. Homozygous Pax6 knockout mice die soon after birth
because Pax6 protein is also required in essential organs, such as the pancreas.
a) In order to study the role of Pax6 in eye development a researcher wants to
generate a mouse that expresses Pax6 everywhere except in its eyes. Describe
how you could construct such a mouse by floxing the gene. Is it possible to
achieve the same end with a transgene? (Hint: think about using cDNA and
RNAI)
b) Suppose you want to create a mouse similar to that in part (a), but one where the
eye cells from Pax6 function has been removed and now express a gene that
specifies a green fluorescent protein (GFP). Marking the cells in this way will
allow the investigators to see the shapes of the Pax6- eye cells more easily than
if they did not express GFP. Diagram a Pax6 gene construct that would enable
the researcher to do this…
Protein levels and mRNA levels for a particualr gene don’t always match. For example, the GCN4 gene in yeast is always producing mRNA, but the Gcn4 protein is only made when the cells are starved.
A. what is one mechanism by which changes in mRNA levels are not always matched by changes in the protein product of that mRNA?
The following diagram is from a paper investigating the role of β4 integrin in breast cancer. They examined the expression of miRNAs (rows) in ten β4 positive and ten β4 negative breast cancers. Each array was performed in triplicate (columns) (Gerson, 2012).
B. β4 integrin is normally involved in attaching epithelial cells to the extracellular matrix and holding them in place, but in cancer, it can be involved in attaching cells to actin and increasing motility as well as in cell signaling. How is β-integrin expression related to miRNA expression? What do you think this means for these breast cancers?
Chapter 18 Solutions
Genetics: From Genes to Genomes
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...
Ch. 18 - The goal of the Knockout Mouse Project is to...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - a. Which genome manipulation technique would you...Ch. 18 - a. Diagram a knockin construct that could have...Ch. 18 - Prob. 16PCh. 18 - Prob. 17PCh. 18 - The transcription factor Pax6 is required...Ch. 18 - Mouse models for human genetic diseases are...Ch. 18 - One way to determine where inside a cell a protein...Ch. 18 - In Problem 5 in Chapter 17, you saw that a SNP...Ch. 18 - Scientists now routinely use CRISPR/Cas9 to make...Ch. 18 - Geneticists are currently considering using...Ch. 18 - a. Figures 18.9 and 18.12 demonstrated methods to...Ch. 18 - Nonhomologous end-joining NHEJ of a double-strand...Ch. 18 - One problem that researchers sometimes encounter...Ch. 18 - Researchers at the University of California at San...Ch. 18 - Prob. 28PCh. 18 - F. Port and S. Bullock at the University of...Ch. 18 - On Fig 18.14, locate the PAM site and identify the...Ch. 18 - Prob. 31PCh. 18 - Prob. 32PCh. 18 - Recall that Leber congenital amaurosis LCA, a form...Ch. 18 - One potential strategy for gene therapy to correct...Ch. 18 - Recently, scientists have used a mouse model for...
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