Concept explainers
a.
To determine:
The diagram of a gene construct that can generate a strain with a particular knockout of histone subunit.
Introduction:
The process of gene targeting can be performed in the yeast cell. The sequence of the genome of the yeast cell is available for the researchers.
b.
To determine:
The steps performed for the generation of mutant yeast.
Introduction:
The process of gene targeting can be easily used for creating yeast strain with a particular knockout mutation in any gene.
c.
To determine:
Whether targeting of gene can be performed in haploid or diploid strain.
Introduction:
The knockout mutation is present in every gene inside the genome of the organism. In the knockout technique, the nonfunctional genes are silenced by replacing them with the functional gene.
d.
To determine:
The conclusion and the explanation of the expected result for H2A gene.
Introduction:
H2A gene in the diploid strain is being knocked out in the diploid strain for the generation of the heterozygote.
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Genetics: From Genes to Genomes
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