Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 6P
This problem concerns a technique called enhancer trapping which scientists first developed in Drosophila. The purpose is to find enhancers in the genome and thereby to identify genes that are active in particular cell types. In enhancer trapping, thousands of fly lines are created, each of which has a single copy of the transgene shown here integrated into a random location in the genome via P element–mediated gene transfer. Note that the transgene has a promoter but no enhancer. (The thick horizontal arrows indicate the inverted repeats at the ends of P elements.)
| a. | How could you identify fly lines in which the transgene integrated next to an enhancer? |
| b. | Describe how you could use this technique to identify genes expressed specifically in the fly wing. |
| c. | Do you think that homozygotes for any of the transgene insertions might have a mutant |
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Geneticists often use ethylmethane sulfonate (EMS) to induce mutations in Drosophila. Why is EMS a mutagen of choice for genetic research? What would be the effects of EMS in a strain of Drosophila lacking functional mismatch repair systems?
In the module, you have learned about P-element mediated transgenesis in Drosophila and the concept of using transgenes to rescue mutant phenotypes. In the figure below, you will see a wild type fly with its natural eye colour and three mutants with their eye colours changed to vermillion, white and rosy, respectively. A schematic of P-element mediated transgenesis (as shown in the lectures) is also included in the figure. Please inspect the schematic carefully and choose which of the following statements is true:
I.
Injection of the white experimental transgene into the vermillion mutant embryo will not change the vermillion mutant phenotype
II.
Injection of the white experimental transgene in the rosy mutant embryo will change rosy eye colour to red (wild type)
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Injection of the white experimental transgene in the white mutant embryo will not change the white mutant phenotype
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The following diagram show what is required for an active promoter of a gene of interest, where:A1 = Activator 1A2 = Activator 2Med = MediatorRep = Repressor
Based on the following data, predict:
Chromatin conformation
Methylation state of the proximal promoter
If protein A1 is present or absent
If protein A2 is present or absent
If the mediator is present or absent
If the repressor is absence or present
If this gene is likely to be transcribed or notPlease note that this is an "all or nothing" bonus question, and no partial credit will be awarded. Selecting all answers will result in zero points awarded. Selecting at least one incorrect answer will result in zero points being awarded.
Question 28 options:
Euchromatin
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Methylated promoter
Unmethylated promoter
Activator A1 present
Activator A1 absent
Activator A2 present…
Chapter 18 Solutions
Genetics: From Genes to Genomes
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...
Ch. 18 - The goal of the Knockout Mouse Project is to...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - a. Which genome manipulation technique would you...Ch. 18 - a. Diagram a knockin construct that could have...Ch. 18 - Prob. 16PCh. 18 - Prob. 17PCh. 18 - The transcription factor Pax6 is required...Ch. 18 - Mouse models for human genetic diseases are...Ch. 18 - One way to determine where inside a cell a protein...Ch. 18 - In Problem 5 in Chapter 17, you saw that a SNP...Ch. 18 - Scientists now routinely use CRISPR/Cas9 to make...Ch. 18 - Geneticists are currently considering using...Ch. 18 - a. Figures 18.9 and 18.12 demonstrated methods to...Ch. 18 - Nonhomologous end-joining NHEJ of a double-strand...Ch. 18 - One problem that researchers sometimes encounter...Ch. 18 - Researchers at the University of California at San...Ch. 18 - Prob. 28PCh. 18 - F. Port and S. Bullock at the University of...Ch. 18 - On Fig 18.14, locate the PAM site and identify the...Ch. 18 - Prob. 31PCh. 18 - Prob. 32PCh. 18 - Recall that Leber congenital amaurosis LCA, a form...Ch. 18 - One potential strategy for gene therapy to correct...Ch. 18 - Recently, scientists have used a mouse model for...
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- expression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use.in an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. will this strategy result in good expression of gene X? why or why not?arrow_forwardThe level of transcription of a gene is tested by creating deletions in the gene as shown in the illustration. These modified genes are tested for their level of transcription: (++) normal transcription levels • (+) low transcription levels (+++) high transcription levels. Which deletion is in an enhancer involved in regulating the gene? Normal gene 2 3 4 ++ Deletion 1 3 4 +++ Deletion 2 1 3 4 ++ Deletion 3 1 3 + Deletion 4 2 4 ++ 4+arrow_forwardA gene, which we will call gene C, can be epigenetically modified in such a way that its expression in some cells is permanently silenced. Describe how you could conduct cell-fusion experiments to determine if a cis- or a trans-epigenetic mechanism is responsible for maintaining the silencing of gene C.arrow_forward
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