Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 17, Problem 6P
Summary Introduction

To analyze:

Describe the variations among general genetic code and those present in the mitochondria of some species. Also discuss selective advantage to the mitochondria code.

Introduction:

Mitochondria are essential organelles that are present in almost all cells in eukaryotic organisms. Mitochondria are called as semi-autonomous organelles because they have their DNA as genetic material which replicate independently and produce their mRNA, tRNA, and rRNA. For the translation of mRNAs, they also have their ribosomes but for many functions, mitochondria depend on nuclear genetic material. Mitochondria are considered to be originated from the endosymbiotic association which suggests that mitochondria are descended from the bacteria survived by endocytosis of the some other eukaryotic cell.

A worldwide (universal) genetic code is a group of rules which facilitate the conversion of information encoded by genetic material (DNA). Minute variations are observed between mitochondrial genetic codes and general genetic codes. I.e. A codon that encodes one amino acid in mitochondria encodes a different amino acid in the general genetic code.

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All mutations in mitochondrial genes ultimately affect (whether directly or indirectly) the key function of mitochondria, which is to make ATP. Why then do mutations in different genes cause different diseases, with specific symptoms?
Components of the TIM complexes, the multi- subunit protein translocators in the mitochondrial inner membrane, are much less abundant than those of the TOM complex. They were initially identified using a genetic trick. The yeast Ura3 gene, whose product is an enzyme that is normally located in the cytosol where it is essential for synthesis of uracil, was modified so that the protein carried an import signal for the mitochondrial matrix. A population of cells carrying the modified Ura3 gene in place of the normal gene was then grown in the absence of uracil. Most cells died, but the rare cells that grew were shown to be defective for mitochondrial import. Explain how this selection identifies cells with defects in compo- nents required for import into the mitochondrial matrix. Why don’t normal cells with the modified Ura3 gene grow in the absence of uracil? Why do cells that are defective for mitochondrial import grow in the absence of uracil?Components of the TIM complexes, the multi-…
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Genetic Analysis: An Integrated Approach (3rd Edition)

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