Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 17, Problem 25P
Summary Introduction

To analyze:

A sea slug Elysia chlorotica acquires chloroplasts by consuming an algal food source, Vaucheria litorea. The ingested chloroplasts in sea slug’s digestive epithelium carry out active photosynthesis for months after ingestion. The algal nuclear genome encodes more than 90% but not all of the proteins needed for chloroplast metabolism. It is supposed that the sea slug actively maintains ingested chloroplasts, supplying them with photosynthetic proteins encoded in the sea slug genome.

Determine whether the sea slug has attain photosynthetic genes by horizontal gene transfer from its algal food source. Write down the steps required, and their plausibility, for heritable endosymbiosis to eventuate.

Introduction:

Elysia chlorotica is a small green slug, which shows kleptoplasty. Kleptoplasty is the phenomenon in which some slugs use chloroplast from the algae. Elysia chlorotica shows the symbiotic association with the chloroplasts of marine Vaucheria litorea.

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Antibiotics such as chloramphenicol, tetracycline, and erythromycin inhibit protein synthesis in bacteria, but have no effect on the synthesis of proteins encoded by eukaryotic nuclear genes. Cycloheximide inhibits the synthesis of proteins encoded by nuclear genes, but has no effect on bacterial protein synthesis. How might these compounds be used to determine which proteins are encoded by mitochondrial and chloroplast genomes?
Explain a mechanism for how a nuclear-encoded protein is targeted to the inner membrane of the mitochondrion.  Explain the mechanism for how a nuclear-encoded protein is targeted to the thylakoid membrane. Describe an experiment that could be conducted to show that there is a targeting system for a nuclear encoded protein that ultimately finds its way to a specific organelle.
Chloroplast genomes have sequence homology to cyanobacteria genomes. (True or false)

Chapter 17 Solutions

Genetic Analysis: An Integrated Approach (3rd Edition)

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