Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 17, Problem 13P

You have isolated ( 1 ) a streptomycin-resistant mutant ( str R ) of Chlamydomonas that maps to the chloroplast genome and ( 2 ) a hygromycin resistant mutant ( hyg R ) of Chlamydomonas that maps to the mitochondrial genome. What types of progeny do you expect from the following reciprocal crosses?

mt + str R hyg S × mt str S hyg R

mt + str S hyg R × mt str R hyg S

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The following results are derived from crosses between Neurospora strain xy and strain ++: Tetrad Class 3 4 ху x+ x+ xy ++ ++ ++ +y +y ху +y 25 ++ 3 124 4 (i) Name the ascus type of each class from 1 to 4 as P, NP or T. (ii) Are genes x and y linked? Explain your answer. (iii) If they are linked, determine the map distance between the two genes. If they are unlinked, provide all the information you can about why you draw this conclusion.
Neurospora of genotype a + c are crossed withNeurospora of genotype + b +. (Here, + is shorthandfor the wild-type allele.) The following tetrads areobtained (note that the genotype of the four sporepairs in an ascus are listed, rather than listing alleight spores):a + c a b c + + c + b c a b + a + ca + c a b c a + c a b c a b + a b c+ b + + + + + b + + + + + + c + + ++ b + + + + a b + a + + + + c + b +137 141 26 25 2 3a. In how many cells has meiosis occurred to yieldthese data?b. Give the best genetic map to explain these results.Indicate all relevant genetic distances, both betweengenes and between each gene and the centromere.c. Diagram a meiosis that could give rise to oneof the three tetrads in the class at the far right inthe list
From a cross between e+ f+ g+ and e− f − g− strains ofNeurospora, recombination between these linkedgenes resulted in a few octads containing the followingordered set of spores:e+ f+ g+e+ f+ g+e+ f − g+e+ f − g+e− f − g−e− f − g−e− f − g−e− f − g−a. Where was recombination initiated?b. Did crossing-over occur between genes e and g?Explain.c. Why do you end up with 2 f+ : 6 f − but 4 e+: 4 e−and 4g+: 4g−?d. Could you characterize these unusual octads as MIor MII for any of the three genes involved?Explain.

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Genetic Analysis: An Integrated Approach (3rd Edition)

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY