Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 14.3, Problem 2TQ
Summary Introduction
To review:
The importance of fixed position of the electron transport proteins within the cell membrane and the consequences if they got loose in aqueous solutions.
Introduction:
Electron transport chain is the series of complexes that consume oxygen and pass electrons from electron donors to electron acceptors by means of various
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The creation of the H+ gradient in the mitochondria that powers ATP synthase is an example of:
a)Diffusion
b)Passive Transport
c)Active Transport
Figure 7.11 Dinitrophenol (DNP) is an "uncoupler"
that makes the inner mitochondrial membrane
"leaky" to protons. It was used until 1938 as a weight-
loss drug. What effect would you expect DNP to have
on the change in pH across the inner mitochondrial
membrane? Why do you think this might be an
effective weight-loss drug?
Intermembrane
space
Mitochondrial
matrix
ATP Synthase
ADP
Inner mitochondrial
membrane
ATP
Figure 7.11 ATP synthase is a complex, molecular machine that uses a proton (H) gradient to form ATP from
ADP and inorganic phosphate (Pi). (Credit: modification of work by Klaus Hoffmeier)
TOM
A given enzyme is able to utilize five different substrates. The Km's have
measured for each substrate. Which substrate is most likely the biochemi
relevant one in the cell? In other words, which one is most likely the pred
vivo substrate for this enzyme? (Choose the one best answer).
Substrate 1 with KM= 4.7 × 10-1 M.
Substrate 2 with Km = 1.5 × 10-2 M.
Substrate 3 with KM = 1.5 x 10-8 M.
×
Substrate 4 with Km= 4.7 × 10-³ M
Substrate 5 with KM = 4. 7 × 10-5 M
Chapter 14 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 14.1 - Prob. 1TQCh. 14.1 - Prob. 2TQCh. 14.2 - Prob. 1TQCh. 14.2 - Prob. 2TQCh. 14.2 - Prob. 3TQCh. 14.3 - Prob. 1TQCh. 14.3 - Prob. 2TQCh. 14.3 - Prob. 3TQCh. 14.4 - Prob. 1TQCh. 14.5 - Prob. 1TQ
Ch. 14.5 - Prob. 2TQCh. 14.6 - Prob. 1TQCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - Prob. 6RQCh. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - Prob. 11RQCh. 14 - Prob. 1TQCh. 14 - Prob. 2TQCh. 14 - Prob. 3TQCh. 14 - Prob. 4TQ
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- Figure 4.15 Cyanide inhibits cytochrome c oxidase, a component of the electron transport chain. If cyanide poisoning occurs, would you expect the pH of the intermembrane space to increase or decrease? What affect would cyanide have on ATP synthesis? Figure 4.15 (a) The electron transport chain is a set of molecules that supports a series of oxidation-reduction reactions. (b) ATP synthase is a complex, molecular machine that uses an H+ gradient to regenerate ATP from ADP. (c) Chemiosmosis relies on the potential energy provided by the H+ gradient across the membrane.arrow_forwardIn the previous question, we identified what products are needed and made at each step of the electron transport chain. In this question, I'd like you to think about what would happen if we applied an herbicide that prevented plant growth by inhibiting some of the pieces in your diagram above. For example, if we applied an herbicide to our plants and it affected the ability of Photosystem II to work, it would prevent he production of oxygen. Let's say we apply an herbicide to our plants and it affects the function of ATP synthase. What product would be inhibited? Group of answer choices ATP NADPH G3P Oxygenarrow_forwardFigure 1 shows a model of the electron transport chain found in the mitochondria of eukaryotes. Based on Figure 1, explain the most likely location of an electron transport chain in Escherichia coli, a prokaryote.arrow_forward
- Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.arrow_forwardA newly identified bacterium is unable to synthesize ubiquinone. A mobile electron carrier called CXC3 is used as a substitute. From the information provided in the table, calculate the delta G knot prime and the Keq value at 298 K for the redox reaction that occurs in this bacterium’s electron transport chain. Explain the impact that using CXC 3 instead of ubiquinol will have on ATP production in the cell. How might this cell adapt to this situation?arrow_forwardDraw a simple word diagram showing the path of electrons through the electron-transport chain.b) On your word diagram below, circle the complexes that pump protons across the inner mitochondrial membrane. Put a box around mobile electron carriers.arrow_forward
- Describe the role of each of the membrane proteins shown in the picture below. Think about what is happening to the electrons and describe how energy is transformed as electrons move along the chain. Part of this involves the formation of the proton gradient. The other part is the explanation of the energetics of electron transport that you investigated above. In other words, the reason that electrons always flow from complex I to complex III to complex IV to oxygen.arrow_forwardWhen electron gradients are created in photosythesis and cellular respiration, the electrons are attatched to a proton, so why does it still have a negative charge?arrow_forwardexplain the relationship between the following structures (cell cytoplasm, mitochondria, krebs-cycle, electron transport chain).arrow_forward
- Photosynthesis and aerobic cellular respiration both rely on electron transport chains to generate ATP. Which of the following does not correctly identify similarities and differences in the ETCs of these processes? a) Electrons delivered to the ETC are used to generate a proton gradient across the membrane b) In photosynthesis, the facilitated diffusion of protons across the membrane generates ATP and glucose molecules; in cellular respiration, this process generates ATP c) In photosynthesis, electrons are delivered to the ETC by NADPH; in cellular respiration, electrons are delivered to the ETC by NADH and FADH2 d) In prokaryotes, active transport moves protons across the cell’s plasma membrane during photosynthesis and cellular respirationarrow_forwardFigure 1 shows a model of the electron transport chain found in the mitochondria of eukaryotes.arrow_forwardDuring cellular respiration in procaryotic cells where is the electron will transport? 1) plasma membrane 2) mitochondria 3) chloroplast 4) nucleusarrow_forward
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