Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 14.3, Problem 1TQ
Summary Introduction

To review:

The function of oxidoreductase in the electron transport chain and the reason for indirect interaction between oxidoreductase and cytochrome complex.

Introduction:

A series of complexes that transfer an electron from electron donors to the electron acceptors through reduction and oxidation reactions (redox reactions), and couple the electron transfer with the proton transfer on the other side of the membrane is known as electron transport chain. This transport chain generates an electrochemical proton gradient that controls the synthesis of ATP (adenosine triphosphate).

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Discuss the role of E286 in the proton transfer carried out by cytochrome c oxidase.  What are the D- and K-pathways? What is the fate of the protons from these two pathways? What is the role of E286? What three mechanisms contribute to the movement of the protons along these pathways?
When pure reduced cytochrome c is added to carefully prepared mito- chondria along with ADP, P, antimycin A, and oxygen, the cytochrome c becomes oxidized, and ATP is formed, with a P/O ratio approaching 1. Why was antimycin A added, and what does this experiment tell you about the location of coupling sites?
Why do the cytochrome electron-transfer processes have different standard reduction potentials, despite the fact that they all utilize the identical iron oxidation–reduction reaction?
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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY