Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 14.2, Problem 2TQ
Summary Introduction
To review:
The simple experiment providing evidence that a proton pump in the bacterial cell membrane drives the efflux of antibiotics such as tetracycline.
Introduction:
A proton gradient is created across the membrane by an essential membrane protein. This pump is known as the proton pump. These proton pumps efflux out hydrogen ions from low concentration medium to high concentration medium. Proton pump inhibits acid secretion and decreases the chances of ulcers in the stomach. Proton pump inhibitors also prevent the negative effect of the antibiotic on the cell membrane of the bacteria.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the practical implication of exponential growth in bacteria?
Why do acid-fast positive bacteria grow more slowly?
The ribosome is the target for many important antibiotics. These drugs must discriminate between bacterial and eukaryotic ribosomes to achieve drug specificity and toxicity. For the two common antibiotics below, what is their mechanism of action and why are they more toxic to bacteria than eukaryotes?
a) Tetracycline
b) Erythromycin
Chapter 14 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 14.1 - Prob. 1TQCh. 14.1 - Prob. 2TQCh. 14.2 - Prob. 1TQCh. 14.2 - Prob. 2TQCh. 14.2 - Prob. 3TQCh. 14.3 - Prob. 1TQCh. 14.3 - Prob. 2TQCh. 14.3 - Prob. 3TQCh. 14.4 - Prob. 1TQCh. 14.5 - Prob. 1TQ
Ch. 14.5 - Prob. 2TQCh. 14.6 - Prob. 1TQCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - Prob. 6RQCh. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - Prob. 11RQCh. 14 - Prob. 1TQCh. 14 - Prob. 2TQCh. 14 - Prob. 3TQCh. 14 - Prob. 4TQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?arrow_forwardWhy do low concentrations of alcohol inhibit Gram-negative bacteria more than Gram-positive bacteria? Explain how the alcohol interacts with: 1) Gram-negative cell wall structures 2) Gram-positive cell wall structuresarrow_forwardIf you isolate a bacteria from the digestive system system of mouse, what would you expect the bacterial response to varying pH to be? What would you likely classify this bacteria as?arrow_forward
- Why might bacteria use quorum sensing to regulate genes needed for virulence? How might this reason be related to the rationale behind using quorum sensing to establish a symbiotic relationship?arrow_forwardWhy might clinical medicine have an interest in understanding bacterial cell division at the molecular level? Explain why a hyperthermophile would probably not be a human pathogen. Describe four factors that may have an influence on the effectiveness of an antimicrobial treatment. Explain why 70% or 80% alcohol is more effective than 100% alcohol in controlling microorganisms.arrow_forwardthe FimH crystal structure contains a nickel ion. Predict what might have happened if a nickel-chelated drug had been used in the bacterial adhesion experiments.arrow_forward
- In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal mediumarrow_forwardWhat cell types would be able to grow on the ECM in the following situations? F- , Hfr , Mix a) Streptomycin was not added to the ECM. b) The ECM contains thiamine. c) The ECM contains all 20 amino acids and all 5 nitrogenous nucleic acid basesarrow_forwardYou perform a Gram staining technique on two bacterial species and observe that one is stained purple while the other is stained pink. Which of the following is consistent with this observation? a) The purple one is likely more resistant to penicillin b) Both bacteria have a cell wall made of mycolic acid c) Their peptidoglycan layers have the same thickness d) They differ in the number of phospholipid membranes e) One has a monolayer of phospholipids while the other has a bilayer of phospholipidsarrow_forward
- In Methicillin resistant bacteria, what have the bacteria developed that allows them to stay alive in the presence of Methicillin?arrow_forwardBacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution a) In your own words give an introduction to the microbial growth and the effect tempature has. b) In your own words explain the importance of the spectrophotometer in regards to microbial growth. c) In your own words give a hypothesis on how bacteria will react to different temperatures.arrow_forwardBringing glucose inside of a bacterium (via group translocation) requires energy. (a) What is the energy used for in this case? (b) When resources are limited for a bacterium, why would the bacterium be willing to spend energy to group translocate glucose?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Microbial Nutrition and Growth; Author: Scientist Cindy;https://www.youtube.com/watch?v=rK3UkyWjkl8;License: Standard YouTube License, CC-BY