If you isolate a bacteria from the digestive system system of mouse, what would you expect the bacterial response to varying pH to be? What would you likely classify this bacteria as?
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- You perform a Gram staining technique on two bacterial species and observe that one is stained purple while the other is stained pink. Which of the following is consistent with this observation? a) The purple one is likely more resistant to penicillin b) Both bacteria have a cell wall made of mycolic acid c) Their peptidoglycan layers have the same thickness d) They differ in the number of phospholipid membranes e) One has a monolayer of phospholipids while the other has a bilayer of phospholipidsThe images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?What phenomenon would you expect to see occurring under a microscope when healthy cells of E. coli bacteria are placed in a dish of distilled water? Briefly explain the underlying mechanism.
- "what is the reason for forming mucoid colonies in some types of bacteria?"You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?In a series of infection experiments, a researcher discovers that the ID50 value for the infectious bacterium Parasiticum mucoides is 2,000, and that the ID50 for another infectious bacterium, Donoteatum thisbacterium, is 150. Given these data, a person exposed to 1,000 bacteria of each type would be more likely to be infected by which bacterium? There is no way to know given the information provided Both infections are equally likely Donoteatum thisbacterium Parasiticum mucoides
- Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?1) The inactivation of Staphylococcus aureus was studied in pomegranate juice heated at 75°- 95°C. The slopes for the inactivation of this bacteria were calculated from the linear lines in semi-log graphical paper. The slope values were presented in Table 1. a) Determine the temperature change needed to inactivate this bacteria by 90%. b) Temperature change to inactivate Bacillus cereus in pomegranate juice by 90% at the same temperature range was 29°F. At 75°-95°C temperature range, which bacteria will be more affected by the temperature changes? Commend on your answer in one sentence Table 1- Slope values for the inactivation of Staphylococcus aureus in pomegranate juice heated at various temperature Temp. (°C) 75 80 85 95 -Slope x 10² (min-¹) 2.222 3.704 5.000 13.158
- You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?Bacillus thuringiensis makes toxins that kill insects. These toxins must be applied several times during the growth season to prevent insect damage. As an alternative to repeated applications, one strategy is to apply bacteria directly to leaves.However,B. thuringiensis does not survive very long in thefield. Other bacteria, such as Pseudomonas syringae, do survive.Propose a way to alter P. syringae so it could be used as an insecticide.Discuss advantages and disadvantages of this approach compared with the repeated applications of the toxins from B. thuringiensis.In Methicillin resistant bacteria, what have the bacteria developed that allows them to stay alive in the presence of Methicillin?