Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 13, Problem 3Q
Summary Introduction
To review:
Whether deoxyribonucleic acid (
Given:
In an experiment, a pulse and chase method were applied to the cells and the DNA extracted from them was subjected to the gel electrophoresis.
Figure 1: DNA bands representing a pulse and chase method.
Introduction:
The duplication of DNA takes place during the S phase of the cell cycle. If the cells are subjected to the medium containing radioactive thymidine during this phase, then the DNA extracted from them can be observed in the gel electrophoresis.
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Below is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers
(1) Which end (5' or 3') of the molecule is here?
(2) Which enzyme is probably functioning here to deal with supercoils in the DNA?
(3) Which enzyme is probably functioning here to unwind the DNA?
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If DNA is replicating in a cell, how does it do this? Describe the mechanism of
replication.
Chapter 13 Solutions
Life: The Science of Biology
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- One characteristic of the DNA molecule is its replication capability. What are the consequences of failures during DNA replication?arrow_forwardDNA replication is vital for successful cell division. Explain the process of DNA replication. Make sure to use the following terms: helicase, S-phase, DNA polymerase and DNA ligase, template, free nucleotides. Use the diagram if it helps you illustrate your points.arrow_forwardBelow is a diagram of DNA replication as currently believed to occur in E. coli. From specific points, arrows are provided that lead to numbers. Answer the questions below relating to the locations specified by the numbers. (02) What end (5’ or 3’) of the molecule is here? (State which) What enzyme is probably functioning here to deal with supercoils in the DNA? What enzyme is probably functioning here to unwind the DNA? What nucleic acid is probably depicted here? What are these short DNA fragments usually called? What enzyme probably functions here to couple these two newly synthesized fragments of DNA? Is this strand the leading or lagging strand? What end (5’ or 3’) of the molecule is here? (State which)arrow_forward
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