Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 13.1, Problem 4R
Summary Introduction
To review:
The experiments utilizing radioactive isotopes that would prove deoxyribonucleic acid (DNA) to be the genetic material and not proteins that are transferred to bacterial cells.
Introduction:
Some bacteria reproduce by various methods, one of them is by conjugation. During this type of reproduction, one of the bacteria carry an ‘F’ factor (genetic material called the plasmid), which is a determinant of donor cell, while the other bacteria does not carry any factor, this being the determinant of a recipient cell. The donor cell or the F+ bacteria, forms a conjugation tube between itself and the recipient cell (F−) in order to transfer the F-plasmid.
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It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation.
a) List all the steps from cell pellet preparation to DNA elution.
b) With which feature can the membrane in the column that comes with the commercial kit bind DNA?
c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?
We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media:
A) Donor and recipient strain mixed together (control - no treatment).
B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells).
The results:
Tubes A, B, and D: HGT was observed.
Tube C: HGT was NOT observed.
Based on this, which type of HGT was occurring?
Conjugation,…
Explain the following statement :
a) initiation of bacteriall DNA replication is an energy requiring process
b) bacterial DNA polymerase can enter the termination sequence but cannot exis
Chapter 13 Solutions
Life: The Science of Biology
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