Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 13, Problem 1TQ
Summary Introduction

To review:

The reason for the presence of glucose catabolism pathways in bacterial habitats even where glucose is scarce.

Introduction:

Bacteria catabolize organic molecules to obtain energy and also for getting substrates that can be used for biosynthesis. Some catabolic substrates are more often used as compared to others because they need less activation energy or few enzymes to dissociate. There are different classes of catabolic substrates, namely carbohydrates, lipids, peptides, and aromatic molecules.

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A “pulse-chase” experiment using 14C-labeled carbon sources is carried out on a yeast extract maintained under strictly anaerobic conditions to produce ethanol. The experiment consists of incubating a small amount of14C-labeled substrate (the pulse) with the yeast extract just long enough for each intermediate in the fermentation pathway to become labeled. The label is then “chased” through the pathway by the addition of excess unlabeled glucose. The chase effectively prevents any further entry of labeled glucose into the pathway.(a) If [1-14C]glucose (glucose labeled at C-1 with 14C) is used as a substrate, what is the location of 14C in the product ethanol? Explain.(b) Where would 14C have to be located in the starting glucose to ensure that all the 14C activity is liberated as 14CO2 during fermentation to ethanol? Explain.
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