Essentials of Genetics (9th Edition) - Standalone book
Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 12, Problem 6PDQ
Summary Introduction

To review:

The coding experiments were done by using repeating copolymers to assign the different triplet codes to its correct amino acids.

Introduction:

Triplet codons are degenerate. It means more than one amino acid can be specified by more than one triplet codons. Wobble hypothesis was postulated by Crick in 1966. This hypothesis was used to predict that the initial two nucleotides of the codon are more critical as compared to the third nucleotide in attracting correct transfer RNA (ribonucleic acid) during the translation process.

Given:

AGG codes for arginine.

The table below shows the data of coding experiments carried out by using repeating copolymers.

Copolymer Codon produced Amino acids in a polypeptide
AG AGA, GAG arg (arginine), glu (glutamine)
AAG AGA, AAG, GAA Lys ( lysine), arg, glu

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In a coding experiment using repeating copolymers, the following data were obtained. Copolymer       Codons Produced           Amino Acids in PolypeptideAG                      AGA, GAG                           Arg, GluAAG                   AGA, AAG, GAA                   Lys, Arg, Glu AGG is known to code for arginine. Taking into account the wobble hypothesis, assign each of the four remaining different triplet codes to its correct amino acid.
The chemical bisulfite transforms cytosine (C) to uracil (U) (U). In DMSO, bisulfite is dissolved. Bisulfite was treated ribosomes. The bisulfite was subsequently removed, and the amount of protein produced was determined using a translation assay. Why do you think we need a DMSO treated control group instead of a group not treated with anything?
The following DNA sequences found on the sense strand belong to the same eukaryotic gene: Sequence 1: 5'-GATTCAATAAAGCTCAGATCGCTCACGTCGCGACTC-3' Sequence 2: 5'-TCCGAGGTCACTAGATACTCGTCGATCGTATAAATG-3' a) Which sequence is likely to be found upstream from the coding sequence? Justify your answer. b) Which sequence is likely to be found downstream from the coding sequence? Justify your answer. c) Which sequence will not be transcribed into an mRNA transcript? Justify your answer.

Chapter 12 Solutions

Essentials of Genetics (9th Edition) - Standalone book

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