To design: An experiment to establish the fact that DNA replication is conservative. Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba .

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Answer 1

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Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

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Using the same experimental setup as in the original Meselson and Stahl experiment, predict the bands (heavy, light or intermediate) that you would observe after one round of replication if DNA polymerase replicated the bacterial genome by the conservative model of replication. Draw and explain your prediction.

Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.

Suppose that replication is initiated in a medium containing moderately radioactive tritiated thymine. After a few minutes of incubation, the bacteria are transferred to a medium containing highly radioactive tritiated thymidine. Sketch the autoradiographic pattern that would be seen for (a) undirectional replication and (b) bidirectional replication, each from a single origin.

Answer 2

Question

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Expert Solution & Answer

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Answer 3

Question

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Expert Solution & Answer

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Check out a sample textbook solution

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Answer 4

Question

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Expert Solution & Answer

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Check out a sample textbook solution

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Answer 5

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Answer 6

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Answer 7

Chapter 11, Problem 26ESP

Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Answer 8

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Answer 9

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

Answer 10

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Answer 11

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Answer 13

Ch. 11 - In the Meselson-Stahl experiment, which of the...Ch. 11 - An alien organism was investigated. When DNA...Ch. 11 - Why might mutations in genes encoding telomerase...Ch. 11 - Although the brother is an immunologically matched...Ch. 11 - Prob. 3CS Ch. 11 - HOW DOWE KNOW? In this chapter, we focused on how...Ch. 11 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 11 - Compare conservative, semiconservative, and...Ch. 11 - Describe the role of 15N in the MeselsonStahl...Ch. 11 - Predict the results of the experiment by Taylor,...

To design: An experiment to establish the fact that DNA replication is conservative. Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba .

Solution Summary: The author explains the semiconservative mode of DNA replication in Vicia faba by labeling DNA with 3H-thymidine using autoradiography.

Concept explainers

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To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Want to see the full answer?

Chapter 11 Solutions

To design: An experiment to establish the fact that DNA replication is conservative. Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba .

Solution Summary: The author explains the semiconservative mode of DNA replication in Vicia faba by labeling DNA with 3H-thymidine using autoradiography.

Concept explainers

Videos

To design: An experiment to establish the fact that DNA replication is conservative. Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

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Chapter 11 Solutions

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.