Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Textbook Question
Chapter 7, Problem 1Q
A researcher has carried out a PAGE experiment to separate membrane proteins, but forgot to include SDS in preparing the sample. How will this affect the results?
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Protein transfer to a membrane from a developed electrophoretic gel can be performed under which of the following conditions?
Can anyone provide a protocol or source for how to easily obtain plasma membrane sheets?
(A) What property of a protein might make it difficult to transfer it from polyacrylamide gel to nitrocellulose? Explain your
reasoning.
(B) What parameter of the gel transfer protocol can be adjusted that might help improve the transfer of these problematic
proteins to the nitrocellulose membrane? Explain your reasoning.
(C) How can we check if proteins have been successfully transferred from the polyacrylamide gel to nitrocellulose?
Chapter 7 Solutions
Becker's World of the Cell (9th Edition)
Ch. 7 - Which functions of a membrane would be compromised...Ch. 7 - Which of the three historical strands forming...Ch. 7 - Lipids form the fluid part of the fluid mosaic...Ch. 7 - A researcher has carried out a PAGE experiment to...Ch. 7 - Proteins can be attached to the membrane in a...Ch. 7 - Functions of Membranes. For each of the following...Ch. 7 - Elucidation of Membrane Structure. Each of the...Ch. 7 - Wrong Again. For each of the following false...Ch. 7 - QUANTITATIVE Gorter and Grendel Revisited. Gorter...Ch. 7 - Prob. 7.5PS
Ch. 7 - QUANTITATIVE Thats About the Size of It. From...Ch. 7 - Temperature and Membrane Composition. Which of the...Ch. 7 - Membrane Fluidity and Temperature. The effects of...Ch. 7 - The Little Bacterium That Cant. Acholeplasma...Ch. 7 - Hydropathy: The Plot Thickens. A hydropathy plot...Ch. 7 - Problem Set Inside or Outside? From Figure 7-22,...Ch. 7 - Prob. 7.12PS
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Make a table with a scale of absorbance and the concentration of protein in Chromatin sample from the following data for excel graph Absorbance=660nm following data are of tubes with concern A =0 B=0.036 C=0.011 D=0.001 E=0.027 F=0.020 G=0.032 H1=0.176 H2=0.183 I1=0.150 I2=0.171 also plot the graph??arrow_forwardIn cells, microtubule assembly depends on other proteins as well as tubulin concentration and temperature. What types of proteins influence microtubule assembly in vivo, and how does each type affect assembly?arrow_forwardYou are interested in the mobility of two different unknown integral plasma membrane proteins—protein X and protein Y—in a cell. In one cell, you fluorescently label all the X proteins. In another cell, you label all the Y proteins. When you perform FRAP analysis on both cells, you find that most of the protein X fluorescence has recovered within 3 minutes, but recovery of the protein Y fluorescence is significantly slower. Even after 10 minutes, only 50% of the protein Y fluorescence has recovered. Describe the FRAP experimental protocol, explain what the results tell you about mobility of the X and Y proteins, and propose a possible explanation for why the mobilities of the two proteins differ.arrow_forward
- Protein structure and function: a) Name two common post-translational modifications of proteins in the cell that will affect their structure/function. b) What are prions? Briefly describe their structure and function. C) Explain the principles of protein folding and significance of urea and Bmercaptoethanol in the experimental procedures addressing this question.arrow_forward7) The figure below shows a 3-barrel protein embedded in a hydrophobic membrane. The central cavity of the B-barrel protein is open to aqueous solvent. Which of the following sequences belongs to one of B-strands that forms part of the B-barrel protein? A) LVMIAVL B) ETSDGHR C) LVIMDRE hydrophobic membrane D) VQLTAKL A hydrophoble membrane water-filled cavityarrow_forwardMicrofilaments work together with the protein blank to cause movement in cells. provide two examples:arrow_forward
- The following questions refer to membrane protein isolation A) What is the difficulty when working with membrane proteins and extracting them from the membrane? B) Why do you need stabilization of membrane proteins? What reagents are used for stabilization? C) What methods are used to determine if your extracted membrane protein (GPCR) is functional? D) What methods are used for the enrichment of surface proteins? E) What 2 methods are used to analyze and identify surface proteins after extraction?arrow_forward7) The figure below shows a B-barrel protein embedded in a hydrophobic membrane. The central cavity of the B-barrel protein is open to aqueous solvent. Which of the following sequences belongs to one of B-strands that forms part of the B-barrel protein? A) LVMIAVL B) ETSDGHR C) LVIMDRE hydrophobic membrane D) VQLTAKL hydrophobic membrane water-filled cavityarrow_forwardElectrophoresis is a method used to sort proteins by their size. Why is a detergent added to the buffer? a) Because SDS forms micelles in which the proteins can be transported through the gel in b) Because SDS lowers the pH of the buffer c) Because SDS denaturs the protein d) Because smaller proteins move more slowly through the gel thenarrow_forward
- What is a CBC? In your own words.arrow_forwardIf there is no helix unwinding proteins and destabilizing proteins present, what would happen?arrow_forwardI am studying pancreatic islet cells and have isolated, cloned, and sequenced a novel protein that you postulate has 4 transmembrane segments. What kind of experiment would allow me to test this hypothesis that there are 4 trans membrane segments?arrow_forward
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