The results expected if the inside-out vesicles are subjected to the GO/ 3 H-BH 4 procedure. Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

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Answer 1

Question

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

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Answer 2

Question

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

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Answer 3

Question

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

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Answer 4

Question

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

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Answer 5

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 6

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 7

Chapter 7, Problem 7.12PS

(a)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the GO/³H-BH₄ procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 8

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 9

(b)

Summary Introduction

To determine: The results expected if the inside-out vesicles are subjected to the LP/¹²⁵I procedure.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 10

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 11

(c)

Summary Introduction

To determine: The conclusions that can be drawn if some of the proteins that become labeled by the LP/¹²⁵I method of part (b) were among those that had been labeled when intact cells were treated such that they were incubated with LP in the presence of ¹²⁵I and the membrane proteins were extracted and analyzed on SDS-polyacrylamide gels.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reverse. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 12

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 13

(d)

Summary Introduction

To determine: A method by which a transmembrane protein can be labeled such that it has ³H on one side and ¹²⁵I on the other side.

Introduction: Erythrocyte membranes can be used to prepare sealed vesicles. These vesicles are prepared such that their orientation is reversed. This means that the side of the erythrocyte membrane which faced the cytoplasm, face outwards in these vesicles.

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 7 - Which functions of a membrane would be compromised...Ch. 7 - Which of the three historical strands forming...Ch. 7 - Lipids form the fluid part of the fluid mosaic...Ch. 7 - A researcher has carried out a PAGE experiment to...Ch. 7 - Proteins can be attached to the membrane in a...Ch. 7 - Functions of Membranes. For each of the following...Ch. 7 - Elucidation of Membrane Structure. Each of the...Ch. 7 - Wrong Again. For each of the following false...Ch. 7 - QUANTITATIVE Gorter and Grendel Revisited. Gorter...Ch. 7 - Prob. 7.5PS

Solution Summary: The author explains that erythrocyte membranes can be used to prepare sealed vesicles.

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