Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Textbook Question
Chapter 7, Problem 7.2PS
Elucidation of Membrane Structure. Each of the following observations played an important role in enhancing our understanding of membrane structure. Explain the significance of each, and indicate in what decade of the timeline shown in Figure 7-3 the observation was most likely made.
- (a) When a membrane is observed in the electron microscope, both of the thin, electron-dense lines are about 2 nm thick, but the two lines are often distinctly different from each other in appearance.
- (b) Ethylurea penetrates much more readily into a membrane than does urea, and diethylurea penetrates still more readily.
- (c) The addition of phospholipase to living cells causes rapid digestion of the lipid bilayers of the membranes, which suggests that the enzyme has access to the membrane phospholipids.
- (d) When artificial lipid bilayers are subjected to freeze-fracture analysis, no particles are seen on either face.
- (e) The electrical resistivity of artificial lipid bilayers is several orders of magnitude greater than that of real membranes.
- (f) Some membrane proteins can be readily extracted with 1 M NaCl, whereas others require the use of an organic solvent or a detergent.
- (g) When halobacteria are grown in the absence of oxygen, they produce a purple pigment that is embedded in their plasma membranes and has the ability to pump protons outward when illuminated. If the purple membranes are isolated and viewed by freeze-fracture electron microscopy, they are found to contain patches of crystalline particles.
Figure 7-3 Timeline for Development of the Fluid Mosaic Model. The fluid mosaic model of membrane structure that Singer and Nicolson proposed in 1972 was the culmination of studies dating back to the 1890s (a–e). This model (f) has been significantly refined by subsequent studies (g and h).
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Transport Across the Cell Membrane Worksheet
1. For each of the following examples, state whether the solution is isotonic, hypertonic or
hypotonic and draw an arrow to indicate which way water will move.
a)
b)
100% H₂O
10% NaCl
0% NaCl
90% H₂O
83
5% salt
10% salt
CELL STRUCTURE AND FUNCTION – EUKARYOTES, PROKARYOTES AND VIRUSES-LABORATORY REPORT SHEET
ACTIVITY 2: CELL MEMBRANES, OSMOSIS AND DIFFUSION
Initial Volume (Vi)
(mL)
Final Volume (Vf)
(mL)
Change in Volume
(Vi – Vf)
(mL)
A
20% sucrose
20% sucrose
92.0 mL
94.0 mL
-2.0 mL
B
40% sucrose
20% sucrose
92.0 mL
92.0 mL
0.0 mL
C
20% sucrose
40% sucrose
92.0 mL
90.0 mL
2.0 mL
Treatment
Percent Change
A
-2%
B
0%
C
2%
Hypothesize which dialysis bags should gain, lose or stay the same in regards to volume. Explain.
Which solution is hypotonic, hypertonic and isotonic?
Explain what the change in volume of the dialysis tube indicated and describe what happened when the volume increased and when the volume decreased.
Explain the relationship between solute concentration and amount of free water or water available for movement.
3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached)
Rank the 3 cell membranes in terms of DECREASING fluidity and explain your ranking based on their FRAP curves, most probable appearance, and composition of the membranes.
Draw the expected image of the surface of the 3 membranes BEFORE photobleaching and AFTER fluorescence recovery as viewed under a fluorescence microscope.
Chapter 7 Solutions
Becker's World of the Cell (9th Edition)
Ch. 7 - Which functions of a membrane would be compromised...Ch. 7 - Which of the three historical strands forming...Ch. 7 - Lipids form the fluid part of the fluid mosaic...Ch. 7 - A researcher has carried out a PAGE experiment to...Ch. 7 - Proteins can be attached to the membrane in a...Ch. 7 - Functions of Membranes. For each of the following...Ch. 7 - Elucidation of Membrane Structure. Each of the...Ch. 7 - Wrong Again. For each of the following false...Ch. 7 - QUANTITATIVE Gorter and Grendel Revisited. Gorter...Ch. 7 - Prob. 7.5PS
Ch. 7 - QUANTITATIVE Thats About the Size of It. From...Ch. 7 - Temperature and Membrane Composition. Which of the...Ch. 7 - Membrane Fluidity and Temperature. The effects of...Ch. 7 - The Little Bacterium That Cant. Acholeplasma...Ch. 7 - Hydropathy: The Plot Thickens. A hydropathy plot...Ch. 7 - Problem Set Inside or Outside? From Figure 7-22,...Ch. 7 - Prob. 7.12PS
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