The following questions refer to membrane protein isolation A) What is the difficulty when working with membrane proteins and extracting them from  the membrane?  B) Why do you need stabilization of membrane proteins? What reagents are used for  stabilization?  C) What methods are used to determine if your extracted membrane protein (GPCR) is  functional?  D) What methods are used for the en

Q: What are the functions of the following steps in the purification and isolation of proteins:…

A: The lysis of cells is the first step in the purification of a cellular protein.

Q: Describe the method used to isolate vesicles for lipid analysis. How is C-Laurdan used to analyze…

A: Introduction: The below answer provides an explanation of two techniques used to study lipid…

Q: How does subcellular centrifugation exactly work? At slower speeds, do the smaller/less dense…

A: Introduction: Subcellular centrifugation is a centrifugation technique that is used in the isolation…

Q: Following on from the last question, what assays could you do to find out if your mitochondria are…

A: Succinate dehydrogenase is an essential enzyme that connects oxidative phosphorylation and the…

Q: Why should the cell density of the inoculum be as small as possible? Why can we not standardize the…

A: The process of introduction of microbes into a nutrient media or other similar environments which…

Q: conditions are required to obtain a porous membrane by the phase inversion  process?

A: Phase inversion is the regulated transition of a polymer from its liquid phase to its solid phase.…

Q: What is the charge of the target cellular structure of positive staining and negative staining?

A: Basic stains are cationic when it is ionized, the chromogen exhibits a positive charge. the positive…

Q: A good way to increase total proteome coverage without using 2D PAGE is to:     "Use two,…

A: Proteins are often separated and recognized using a variety of separation techniques such as size…

Q: What type of companion cell is commonly known for adopting polymer trapping model and provide…

A: The companion cells are specialized parenchyma cells that are present in the phloem tissues of the…

Q: What is G-banding? O A) Most commonly used banding method B) Uses giemsa dye O C) Produces…

A: A chromosome contains the genetic material, it is present in the nucleus in condensed form.

Q: Which method for determining protein structure involves cleaving proteins into smaller polypeptides…

A: Protein structure refers to the three-dimensional arrangement of amino acid residues in a protein…

Q: 1) both streak plating and pour plating produce isoluated colonies. What is the underlying…

A: Hello there! Since you have posted multiple questions and not mentioned which one of them to be…

Q: You have a cell suspension in an isotonic medium. Explain in detail what would happen when you add:…

A: Urea mannitol acts at a hyperosmolar agent. Both mannitol and hypertonic saline are established as…

Q: What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?

A: The formation of new blood vessels from pre-existing vessels or endothelial cell progenitors is…

Q: What is the purpose of osmosis lab?

A: Osmosis is defined as..

Q: Mild detergents, like Triton X-100, would be the best for isolation of peripheral proteins from the…

A: The cell membrane is a structure which protects the cell and acts as a barrier and transports the…

Q: What are the similarities and differences between membrane protein isolation techniques for…

A: A variety of functions essential to the survival of organisms are performed by membrane proteins:…

Q: What do the following abbreviations mean: DdDp, RdDp, DdRp, RdRp. Which could be provided by cells?

A: Answer: Introduction: RNA-dependent RNA polymerase (RdRp) is also called as RNA replicase, it is a…

Q: What are the disadvantages of the membrane filter method?

A: Filtration is the process of removing unwanted particles from a fluid. This includes the removal of…

Q: subcellular fractionatio

A: Centrifugation:  Molecules with different densities are separated by rotating them in a solution…

Q: How can the foreign protein be modified to facilitate its purification?

A: Protein purification is a progression of cycles proposed to confine one or a couple of proteins from…

Q: Electrophoresis is a method used to sort proteins by their size. Why is a detergent added to the…

A: Electrophoresis  It is a biochemical technique to separate charged molecules under the influence of…

Q: Proteins secreted by bacteria can be used as biomarkers for diagnostics. Give a concise account of…

A: The identification of protein antigens from pathogens can serve as a diagnostic tool. Several…

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: If a scientist drops an animal cell into a solution that contains 10 times more salt than is found…

A: A solution is made up of solute plus solvent . Solution can be :- A ) Hypertonic  B ) Hypotonic  C )…

Q: What are the best methods for isolation of plasma membrane (PM) proteins from mammalian cells?

A: Mammalian cells are the eukaryotic cells obtained from a mammal such as a human. Cells can be from…

Q: 4- Mitotic spindle is observed with: a) O Keratin filaments b) O Actin filaments C)O Cytoplasmic…

A: The growth and development of organisms are attributed to the unique ability of the cell to divide…

Q: What are the steps to the formation of a clathrin-coated vesicle?

A: Transport is an act in which the water, ion, chemical molecules are move outside or inside a…

Q: The cytoskeleton of eukaryotic cells would be best visualized by a) scanning electron microscopy O…

A: Microscope is used to magnify the images that can't be seen by naked eyes.

Q: List three biomaterials that are used in cancer diagnosis and therapeutic. b) List three…

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Q: What is the difference and compare the light source and path of light of a immunofluorescence…

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Q: 1. What is the % of glucose and sodium outside the cell? 2. What is the % of water outside the cell?…

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Q: Find a method that uses some form of HPLC for the analysis of proteins. What was the stationary…

A: Protein analysis by chromatography is a widely used technique for separating, identifying, and…

Q: The SDS-PAGE gel matrix used to separate proteins by electrophoresis is composed of: A)…

A: Biological molecules are separated using the gel electrophoresis which is based on size and other…

Q: Which fractions measured from your gel filtration experiment had the most and least amount of…

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Q: You wish to identify the presence of a specific protein inside the cell. What microscopy technique…

A: Microscopy is a scientific technique that involves the use of microscopes to observe and study…

Q: After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code…

A: Protein is a polymer formed from amino acid joined by peptide bond. It is abundantly found in the…

Q: What cell types would be able to grow on the ECM in the following situations? F- , Hfr , Mix   a)…

A: Introduction  The ECM (extracellular matrix) is a complex mixture of proteins, glycoproteins, and…

Q: (3) What is a protein database? Give examples (and links) of some protein database

A: Bioinformatics is described as the application of tools of computation as well as the analysis to…

Q: What are the requirements of a laboratory dedicated to animal cell culture experiments?

A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.