Biological Science (6th Edition)
6th Edition
ISBN: 9780321976499
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Emily Taylor, Greg Podgorski, Jeff Carmichael
Publisher: PEARSON
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Chapter 4, Problem 14PIAT
QUANTITATIVE In 1951, Erwin Chargaff was accumulating data on the molar ratios of
| Molar Ratios in DNA Preparations | |||||
|---|---|---|---|---|---|
| Source | A:G | T:C | A:T | G:C | purines:pyrimidines |
| Ox | 1.29 | 1.43 | 1.04 | 1.00 | 1.10 |
| Human | 1.56 | 1.75 | 1.00 | 1.00 | 1.0 |
| Chicken | 1.45 | 1.29 | 1.06 | 0.91 | 0.99 |
| Salmon | 1.43 | 1.43 | 1.02 | 1.02 | 1.02 |
| Wheat | 1.22 | 1.18 | 1.00 | 0.97 | 0.99 |
| Yeast | 1.67 | 1.92 | 1.03 | 1.20 | 1.0 |
Source: Chargaff, E. 1951. Federal Proceedings 10" 654–659.
Compare the molar ratios presented from each organism tested and between different organisms. Explain how these data could be used to show that Levene'stetranucleotide model is incorrect. What do they imply about the primary structure of DNA in different organisms?
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Propose an experiment that would test the importance of ionic strength in the determination of DNA melting temperatures. Be specific.
Each group will be provided with 20 g double-stranded DNA oligomer in STE buffer (0.1M NaCl/ Tris/ 10 mM EDTA, pH 7.4). The sequence of one strand of the oligomer is:
5’-AATGGTACGTATACTTTAT-3’
- show a table of % denaturation of DNA at different temperatures, using the equation provided in “Calculation”
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Show the calculations for the preparation of 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions via serial dilution from a 0.1% stock solution.
Prepare 10.0 mL of 0.10% standard DNA solution using acetate buffer pH 4.6 as solvent.
Prepare four different concentrations of the 0.10% standard DNA solution from step 1 via serial dilution to obtain 0.01%, 0.001%, 0.0001%, 0.00001% standard DNA solutions.
Obtain seven clean and dry test tubes. Place 1.50 mL solutions in each tube as follows:
Table 2.1. Set-up for the diphenylamine assay.
Test tube #
Content
1 (blank)
acetate buffer pH 4.6
2
0.10% standard DNA
3
0.01% standard DNA
4
0.001% standard DNA
5
0.0001% standard DNA
6
0.00001% standard DNA
7
DNA extract from Part I
Add 3.50 mL diphenylamine reagent to each tube, swirl each tube to thoroughly mix the contents and heat for 10 mins in a boiling water bath. Cool immediately under tap water.
Read absorbances at 595 nm.…
Chapter 4 Solutions
Biological Science (6th Edition)
Ch. 4 - What are the four nitrogenous bases found in RNA?...Ch. 4 - 2. What determines the primary structure of a DNA...Ch. 4 - 3. Which of the following describes the synthesis...Ch. 4 - Prob. 4TYKCh. 4 - Prob. 5TYUCh. 4 - Prob. 6TYUCh. 4 - What would be the sequence of the strand of DNA...Ch. 4 - 8. According to the RNA world model, a ribozyme...Ch. 4 - Make a concept map (see BioSkills 12 ) that...Ch. 4 - Prob. 10TYPSS
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