Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 27.3, Problem 4TQ
Summary Introduction
To review:
The prediction of the drug resistance
Introduction:
A genome of an organism is its complete set of deoxyribonucleic acid (DNA) including all its genes. Genomics is a field of biology that focuses on the structure-function, editing, and mapping of the genome. Recent advances in genomics have revolutionized discovery-based research to understand even the most complex biological systems.
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Chapter 27 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 27.1 - Prob. 1TQCh. 27.1 - Prob. 2TQCh. 27.1 - Prob. 3TQCh. 27.1 - Prob. 4TQCh. 27.2 - Prob. 1TQCh. 27.2 - Prob. 2TQCh. 27.2 - Prob. 3TQCh. 27.3 - Prob. 1TQCh. 27.3 - Prob. 2TQCh. 27.3 - Prob. 3TQ
Ch. 27.3 - Prob. 4TQCh. 27.4 - Prob. 1TQCh. 27 - Prob. 1RQCh. 27 - Prob. 2RQCh. 27 - Prob. 3RQCh. 27 - Prob. 4RQCh. 27 - Prob. 5RQCh. 27 - Prob. 6RQCh. 27 - Prob. 7RQCh. 27 - Prob. 8RQCh. 27 - Prob. 9RQCh. 27 - Prob. 10RQCh. 27 - Prob. 11RQCh. 27 - Prob. 12RQCh. 27 - Prob. 13RQCh. 27 - Prob. 14RQCh. 27 - Prob. 15RQCh. 27 - Prob. 16RQCh. 27 - Prob. 17RQCh. 27 - Prob. 1TQCh. 27 - Prob. 2TQCh. 27 - Prob. 3TQCh. 27 - Prob. 4TQ
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- Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardRemember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?arrow_forwardIn Biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. (i) State the THREE (3) important regions of the plasmid. Elaborate your answer. (ii) Besides plasmids, name TWO (2) other commonly used vectors in Biotechnology.arrow_forward
- How had DNA Microarray Technology revolutionized the study of gene activity? a) gene expression in cells can now be studied b) the proteins produced by genes have been discovered c) many genes can be studied at the same time d) all of the proteins produced in a cell can now bearrow_forwardWhy is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?arrow_forwardWhich of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).arrow_forward
- What is molecular pharming? Compared with the production of proteins by bacteria, why might it be advantageous?arrow_forwardThe idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)arrow_forwardHow does bisulfite treatment coupled with PCR enable the identification of DNA methylation? And, How could a Southern blot be used to determine if a given DNA sequence contains methylated CpGs in paper "Amyloid-β Alters the DNA Methylation Status of Cell-fate Genes in an Alzheimer's Disease Model." Taher, N., McKenzie, C., Garrett, R., Baker, M., Fox, N., & Isaacs, G. D. (2014). Amyloid-β alters the DNA methylation status of cell-fate genes in an Alzheimer’s disease model. Journal of Alzheimer’s Disease: JAD, 38(4), 831–844. https://doi.org/10.3233/JAD-131061arrow_forward
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