The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique. Introduction: FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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Chapter 25.7, Problem 1TQ

Summary Introduction

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The identification of chemical inhibitors of type II, IV or VI bacterial secretion systems by the use of FRET/beta-lactamase technique.

Introduction:

FRET (fluorescence resonance energy transfer) is a mechanism of energy transfer between two fluorophores. It can be used to detect the host cells that are targeted by a bacterial pathogen. The pathogen targeted host cells exhibit fluorescence due to FRET and can be sorted by flourescence activated cell sorter (FACS).

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