Genetics: Analysis and Principles
Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 24.1, Problem 3COMQ

For the method of RNA sequencing (RNA-Seq), which of the following is the correct order of steps?

a. Isolate RNAs, synthesize cDNAs, fragment RNAs, sequence cDNAs, align cDNA sequences

b. Synthesize cDNAs, sequence cDNAs, isolate RNAs, fragment RNAs, align cDNA sequences

c. Isolate RNAs, fragment RNAs, synthesize cDNAs, sequence cDNAs, align cDNA sequences

d. Synthesize cDNAs, isolate RNAs, fragment RNAs, sequence cDNAs, align cDNA sequences

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What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?
Explain:a. functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomalRNA (rRNA) species-specific sequences.b. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe.
Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing.    A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing.   B. Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons.   C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing.   D. The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer.   E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency.   F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License