what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the genome sequence.

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: What is the main reason for using a cDNA library rather than a genomic library to isolate a human…

A: Introduction :- cDNA stands for "complementary DNA". It is a type of DNA that is synthesized from…

Q: Why is genome database and annotation relevant to Genome sequencing?   Are there any examples?

A: Genome: the set of genes. Genome database: it is the collection of all information on the human…

Q: What is an advantage of making a cDNA library rather than a genomic library?

A: DNA library or the gene library is the collection of DNA sequences from different organisms. These…

Q: Discuss how CRISPR-cas functions as a genome editing tool.

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: What are the differences between a DNA library and acDNA library?

A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: what are the purpose of sequence alignment? define local alignment and global alignment?

A: Alignments are an effective method for comparing similar DNA or protein sequences. DNA molecules are…

Q: What can serve as footprints to identify genome sites that are or have been occupied by transposable…

A: A genome is a collection of an organism's genetic information.

Q: Explain Two strategies for assembling whole genome sequences.

A: All the genetic information of an organism is known as its genome. Genome assembly is defined as the…

Q: How do shotgun and directed sequencing differ? Why is coverage an important issue?

A: Shotgun sequencing and directed sequencing are two different approaches in the field of DNA…

Q: what is the main purpose of performing the bioinformatics analysis of 16s rRNA genes lab?

A: Bioinformatics:  The application of computation and analysis tools to the capture and interpretation…

Q: A.Evaluate the TWO (2) typical forms of research design (quantitative & qualitative) in both time…

A: Physical Activity --Introduction -- Regular physical activity is for healthy and for older people…

Q: Digrammatically represent the experimental steps in cloning and expressing an human gene (say the…

A: The method of DNA cloning is used to produce the multiple identical copies of the specific template…

Q: Why the DNA sequencing alone is often not sufficient to produce a genome assembly of desired…

A: DNA sequencing is basically the process by which the nucleic acid sequence can be determined. the…

Q: A new technique for rapidly determining the nuclesome structure for regions of chromatin is called…

A: In the field of molecular biology, understanding the mechanisms underlying gene expression…

Q: principles,  advantages disavantages of DNA and RNA microarrays  and Taqman SNP analysis and their…

A: SNP stands for single nucleotide polymorphisms. It is a polymorphisms in which variation in DNA…

Q: What is meant by “proofreading” with respect to DNA polymerase?

A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…

Q: Do not use chatgpt.

A: Goals of human genome project:Obtain a physical map of genome- genome; this allows the rough…

Q: How can the Ames test distinguish mutagens that cause small insertions/deletions from those that…

A: *Ames test  a biological assay to find the mutagenic potential of an chemical compound which…

Q: What are the possible bioethical issues that gene editing tools may encounter?

A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…

Q: What is a repetitive element in genomics?  What are the types of repetitive elements?  What is their…

A: The amount  of DNA present in a cell of a person is called as genome. DNA is made up of nucleotides…

Q: Consider a genome whose length is 1000 bp. "Shotgun" sequencing techniques are applied to the…

A: Shotgun sequencing is an important tool in modern genomics that allows researchers to interpret the…

Q: Why the sequence alignment is extremely important in designing a construct for developing a…

A: Sequence alignment It is defined as the way of arranging the sequences of DNA, RNA or protein to…

Q: Discuss the bioinformatics approaches that can be used to identifya protein-encoding gene.

A: The process of recognizing the protein-encoding gene is crucial and has numerous applications. Such…

Q: Exaplain the two methods to study the contents of transcriptomes which are microarray analysis and…

A: Transcriptome analysis may be used to find novel genes and isoforms that are not already cataloged…

Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…

A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: What types of cells are used for cloning rDNA?

A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: Some synthetic biologists have proposed creating an entirely new, freeliving organism with a minimal…

A: A genome is the entire DNA collection of an organism, including all genes. All the details needed to…

Q: what are similarities and differences between chain-termination and reversible terminator…

A: Gene sequencing  aids in the development of gene therapy, a form of treatment that replaces faulty…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: Who developed the sequencing-bysynthesis (SBS) approach? & When it was firstly used ?

A: Sequencing by synthesis (SBS), an Illumina sequencing method, is a commonly used next-generation…