Place the steps necessary to perform RNA-Seq in the correct order. Drag the text blocks below into their correct order. Reset MAAAAAAAAAKK Compare sequences to known genome sequence. Create cDNA using reverse transcription with primers complementary to linkers. Attach linkers to the ends of the RNAs. Perform next-generation DNA sequencing. Isolate RNA from cells or tissues of interest. Fragment the RNAs.

After ovulation, the mature follicles collapses, taking on a yellowish appearance to become the _________.     Corona radiata   Graafian follicle   Corpus luteum   Zona pellucida   Degenerating follicle

You have assembled a new prokaryotic genome and are annotating the genes.  If you were to search for highly conserved sequences necessary for normal gene expression, what type of annotation are you doing? Group of answer choices ad hoc annotation homology based annotation preliminary annotation ab initio annotation

What is the enzyme used to create DNA complementary to mature mRNA? Group of answer choices ligase topoisomerase DNA polymerase reverse transcriptase RNA polymerase transposase

Use this information to match the list of probes to the 3 samples below: You have isolated several DNA sequences from a variety of mouse tissues. You have labeled each one of them with a radioisotope and will use them as probes on blots of several DNA and RNA somples. Below are a list of all the probes you generated (probes A through E) and a list of all the DNA and RNA samples that you will analyze (Samples 1 through 3) Beside each sample, write the letters corresponding to oll the probes that will bind to a complementary sequence in that sample. These responses are graded all or nothing! List of probes Probe A: promoter sequence of a gene that is only expressed in the nervous system Probe B: promoter sequence of one of the genes encoding a ubiquitously expressed histone protein Probe C: coding sequence of a gene that is only expressed in the nervous system Probe D: coding sequence of one of the genes encoding a ubiquitously expressed histone protein Probe E: intron of a gene that is…

Transcriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment.   (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesis

You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR products

As part of the genomic analysis of "Maxbacterium", one of the small DNA circles was digested with EcoRI and Hind III restriction endonuclease enzymes. Electrophoresis of the resulting digestion fragments is shown in the gel below to help student groups construct the restriction map in their breakout room. Size of EcoR1 HindIII EcoR1 + Fragment digest digest HindIII digest Origin 17kb 10kb 7kb 6kb 1kb Based on the banding pattern and constructed map which of the following statements is TRUE? O A. The largest fragment in the restriction map is 17KB. O B. The information provided is enough to quantify the amount of DNA in the gel. O C. The migration on the gel depends on the proportion of AT pairs in the DNA. O D. The constructed restriction map has three cut sites.