Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 23.2, Problem 1COMQ
Summary Introduction
Introduction:
Fluorescence, in the situ hybridization (FISH) technique, is used to map the location of certain genes. In this technique, the chromosomal deoxyribonucleic acid (DNA) is first denatured, and then, a probe is added to it. FISH is used for the detection of various diseases such as, phenylketonuria and various cancerous diseases which, are caused by mutation of genes.
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Chapter 23 Solutions
Genetics: Analysis and Principles
Ch. 23.1 - Prob. 1COMQCh. 23.2 - Prob. 1COMQCh. 23.3 - A molecular marker is a _____ found at a specific...Ch. 23.3 - 2. Which of the following is an example of a...Ch. 23.3 - To map the distance between molecular markers via...Ch. 23.4 - 1. What is a contig?
a. A fragment of DNA that...Ch. 23.4 - A vector that can carry a large fragment of...Ch. 23.4 - 3. Chromosomal walking is a method of _____ in...Ch. 23.5 - Prob. 1COMQCh. 23.5 - Prob. 2COMQ
Ch. 23.5 - 3. A prokaryotic genome is about 4 million bp in...Ch. 23.6 - Metagenomics is aimed at a. determining the...Ch. 23 - 1. A person with a rare genetic disease has a...Ch. 23 - For each of the following, decide if it could be...Ch. 23 - Which of the following statements about molecular...Ch. 23 - 1. Is each of the following a method used in...Ch. 23 - Prob. 2EQCh. 23 - Prob. 3EQCh. 23 - The cells from a persons malignant tumor were...Ch. 23 - 5. Figure 23.2 describes the technique of FISH....Ch. 23 - Explain how DNA probes with different fluorescence...Ch. 23 - 7. A researcher is interested in a gene found on...Ch. 23 - Prob. 8EQCh. 23 - Prob. 9EQCh. 23 - Prob. 10EQCh. 23 - Prob. 11EQCh. 23 - Prob. 12EQCh. 23 - In the Human Genome Project, researchers have...Ch. 23 - 14. Take a look at question 3 in More Genetic...Ch. 23 - 15. Place the following stages of a physical...Ch. 23 - 16. What is an STS? How are STSs generated...Ch. 23 - 17. Four cosmid clones, which we will call cosmids...Ch. 23 - A human gene, which we will call geneX, is located...Ch. 23 - 19. Describe how you would clone a gene by...Ch. 23 - 20. A bacterium has a genome size of 4.4 Mb. If a...Ch. 23 - 21. Discuss the advantages of next-generation...Ch. 23 - Prob. 22EQCh. 23 - Prob. 23EQCh. 23 - What is a molecular marker? Give two examples....Ch. 23 - Which goals of the Human Genome Project do you...
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- What is the role of Sodium Iodidee in DNA isolation? A. Solubilization of phospholipids B Precipitation of DNA C Destroy hydrogen bonding network in solvent D Precipitation of cel wall and cell membranearrow_forwardThe technique of fluorescence in situ hybridization involves theuse of a that hybridizes to a .a. radiolabeled probe, band on a gelb. radiolabeled probe, specific site on an intact chromosomec. fluorescent probe, band on a geld. fluorescent probe, specific site on an intact chromosomearrow_forwardThe percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genesarrow_forward
- The Hershey-Chase experiment, in which bacteria were infected with radioactively labeled bacterial virus, provided evidence that DNA carries genetic information by showing that A. the bacterial virus injected its 35S-labeled proteins into the cell. B. blender treatment after infection with virus prevented the production of progeny virus particles. C. the production of progeny virus required the injection of viral DNA into the cell. D. 32P-labeled bacterial virus could not produce progeny virus after blender treatment.arrow_forwardYou used agarose gel electrophoresis to separate DNA fragments of different size and the experiment worked well. However, you wanted to re run the experiment but this time you made the gel with a higher percentage of Agarose. How might this affect your results compared to the first run? a. There would be no difference between the runs since it is the current, not the agarose that causes migration. b. The higher concentration of agarose would cause the DNA to break apart. c. You can't predict how the concetration of agarose would affect migration. d. The DNA fragments would migrate further down the gel than they did the first time. e. The DNA fragments wouldn't migrate as far down the gel as they did the first time.arrow_forwardWhen you collect your cheek cells and prepare the DNA, is it really a problem that bacteria and food bits are in the sample too? A Yes, it is contamination, and any kind of contamination is a problem. B No, because food bits and bacteria do not contain human chromosome 16. Only contamination with human DNA of another individual would confuse the results.arrow_forward
- Which of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.arrow_forwardInformation obtained by microscopic analysis of stained chromosomes is used in: a. radiation hybrid mapping b. sequence mapping c. RFLP mapping d. cytogenetic mappingarrow_forwardLampbrush and polytene chromosomes are characteristic because of the fact that A. nucleosomes are 3-dimensionally distinct from "normal" chromosomes B. microscopy shows they have regions with loosely bound DNA near tightly bound regions C. multiple centromeres were discovered on their linear sequence by in situ hybridization D. DNAse is unable to cleave the linkage between strandarrow_forward
- How can you detect 1 small, very specific, piece of DNA (eg: a gene), among THOUSANDS of other pieces or sequences of DNA from nuclear chromosomes? a. DNA hybridization b. RNA hybridization c. Use of a radioactive or fluorescent probe d. Colony blotting and hybridization e. Synthetic primers and PCR f. Any of the above methodsarrow_forwardWhich lab technique would you use for each of the following: a.Break Open Cell Membrane b. Remove Cell Debris c.Amplify Region of Interest d. Analyze DNA for SNP Variationarrow_forwardWhen working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel flat on the table without any buffer solution B. Not changing your pipette in between DNA samples C. Not shaking the chamber after the DNA has been loaded D. Loading two DNA samples per a single wellarrow_forward
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