Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 23, Problem 5EQ
Figure 23.2 describes the technique of FISH. Why is it necessary to fix the cells (and the chromosomes inside of them) to the slides? What does it mean to fix them? Why is it necessary to denature the chromosomal DNA?
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Chapter 23 Solutions
Genetics: Analysis and Principles
Ch. 23.1 - Prob. 1COMQCh. 23.2 - Prob. 1COMQCh. 23.3 - A molecular marker is a _____ found at a specific...Ch. 23.3 - 2. Which of the following is an example of a...Ch. 23.3 - To map the distance between molecular markers via...Ch. 23.4 - 1. What is a contig?
a. A fragment of DNA that...Ch. 23.4 - A vector that can carry a large fragment of...Ch. 23.4 - 3. Chromosomal walking is a method of _____ in...Ch. 23.5 - Prob. 1COMQCh. 23.5 - Prob. 2COMQ
Ch. 23.5 - 3. A prokaryotic genome is about 4 million bp in...Ch. 23.6 - Metagenomics is aimed at a. determining the...Ch. 23 - 1. A person with a rare genetic disease has a...Ch. 23 - For each of the following, decide if it could be...Ch. 23 - Which of the following statements about molecular...Ch. 23 - 1. Is each of the following a method used in...Ch. 23 - Prob. 2EQCh. 23 - Prob. 3EQCh. 23 - The cells from a persons malignant tumor were...Ch. 23 - 5. Figure 23.2 describes the technique of FISH....Ch. 23 - Explain how DNA probes with different fluorescence...Ch. 23 - 7. A researcher is interested in a gene found on...Ch. 23 - Prob. 8EQCh. 23 - Prob. 9EQCh. 23 - Prob. 10EQCh. 23 - Prob. 11EQCh. 23 - Prob. 12EQCh. 23 - In the Human Genome Project, researchers have...Ch. 23 - 14. Take a look at question 3 in More Genetic...Ch. 23 - 15. Place the following stages of a physical...Ch. 23 - 16. What is an STS? How are STSs generated...Ch. 23 - 17. Four cosmid clones, which we will call cosmids...Ch. 23 - A human gene, which we will call geneX, is located...Ch. 23 - 19. Describe how you would clone a gene by...Ch. 23 - 20. A bacterium has a genome size of 4.4 Mb. If a...Ch. 23 - 21. Discuss the advantages of next-generation...Ch. 23 - Prob. 22EQCh. 23 - Prob. 23EQCh. 23 - What is a molecular marker? Give two examples....Ch. 23 - Which goals of the Human Genome Project do you...
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- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardHow to prepare chromosomes for SEM Imaging?arrow_forwardThe microscope image above shows the human chromosomes from a white blood cell. To create the image, researchers put cells in culture under conditions that encourage the cells to divide. They bathed the cells in a hypotonic (low salt) solution, which caused the cells to swell until their plasma membrane burst open. They "squashed" the chromosomes to spread them out, and stained them with a dye to make them visible under the microscope. Human chromosomes are numbered from longest (1) to shortest (22) plus the sex chromosomes X and Y. In the image chromosome 1 is about 7 micrometers. Answer the following questions. 1) What word(s) in the description above indicates that the chromosomes are not from a cell undergoing meiosis? 2) Based on the size, shape and appearance of the chromosomes in the image, in what cell cycle stage was the cell that the chromosomes came from? How can you tell? 3) Does the image suggest that centromere sequences are always located in the middle of a…arrow_forward
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- A scientist collects cells at various points in the cell cycle and isolates DNA from them. Using density-gradient centrifugation, she separates the nuclear DNA and mtDNA. She then measures the amounts of mtDNA and nuclear DNA present at different points in the cell cycle. On the following graph, draw a line to represent the relative amounts of nuclear DNA that you expect her to find per cell throughout the cell cycle. Then, draw a dotted line on the same graph to indicate the relative amounts of mtDNA that you would expect to see at different points throughout the cell cycle.arrow_forwardDescribe the technique of chromosome painting, and explain why it is used.arrow_forwardExplain how electrophoresis separates DNA strands. How is a DNA fingerprinting test interpreted?arrow_forward
- The primary benefit of recombination is: Activity of mobile genetic elements Meiotic recombination DNA double-strand break repair Movement of viruses between lytic and prophage phases Bacterial control of gene expressionarrow_forwardwe done an experiment where we extracted the DNA from strawberry, grape, and peas. With the strawberries, we were able to find its DNA in the solution very easily because it was floating on the top. But for the grapes we were only able to see very small clumps which we weren't able to collect. And for the peas, we weren't able to see anything or collect any of their DNA. all I need is for help with the CER(claim, evidence, and reasoning) thank you!!arrow_forwardPhysical maps are constructed by using a chromosome walking technique.a) Trueb) Falsearrow_forward
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