Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
Question
Book Icon
Chapter 20, Problem 8TYU
Summary Introduction

To explain: The cloning of the human crystallin gene to obtain a sufficient amount of the human crystallin protein.

Concept introduction:

Protein encoding genes express themselves and form functional gene products in the form of proteins. This is known as gene expression. Generally, polymerase chain reaction (PCR) is used for the amplification of the gene sequence of interest and to synthesize its multiple copies. This gene fragment is then inserted into the expression vector to synthesize the protein encoded by the gene.

Blurred answer
Students have asked these similar questions
Give typed full explanation  there are about 28,000 copies of zinc finger domains in the human genome, most of them as constituents of transcribed genes. This is a result of what process? Retro transposition of mobile sequences Evolutionary conservation, exon duplication and exon shuffling Evolutionary conversion of leucine zipper, helix-turn-helix, and helix-loop-helix domains into zinc finger domains Evolutionary selection for proteins that interfere with nucleosome packing Genes that “jump” with the help of a transposase.
. Let’s say that you have incredible skill and can isolate the white and red patches of tissue from the Drosophila eyes shown in Figure 12-24 in order to isolate mRNA from each tissue preparation. Using your knowledge of DNA techniques from Chapter 10, design an experiment that would allow you to determine whether RNA is transcribed from the white gene in the red tissue or the whitetissue or both. If you need it, you have access to radioactive white-gene DNA
Suppose that you have just graduated from college and have started working at a biotechnology firm. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; however, the mouse gene for prolactin has been cloned, and the amino acid sequence of mouse prolactin is known. Briefly explain two different strategies that you might use to find and clone the pig gene for prolactin.
Knowledge Booster
Background pattern image
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Text book image
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning