Concept explainers
(a)
To identify: The genes expressed in a specific type of brain cell.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(b)
To isolate: The neurotransmitter gene expressed in brain cells.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(c)
To produce: Multiple copies of the gene encoding for neurotransmitter protein.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
(d)
To produce: Large quantities of the neurotransmitter.
Introduction:
A particular gene can be identified through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to a gene of interest are to be identified and reverse transcribed to form cDNA. This sequence can be identified through RT-PCR, DNA microarray analysis, and RNA sequencing. To study a particular protein, multiple copies of the gene fragment encoding that protein are synthesized through polymerase chain reaction (PCR). These gene fragments are then inserted into the expression vector to synthesize a large quantity of proteins encoded by the gene in a process known as gene cloning.
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Campbell Biology
- Beadle and Tatum (a) predicted that tRNA molecules would have anticodons (b) discovered the genetic disease alkaptonuria (c) showed that the genetic disease sickle cell anemia is caused by a change in a single amino acid in a hemoglobin polypeptide chain (d) worked out the genetic code (e) studied the relationship between genes and enzymes in Neurosporaarrow_forwardmRNA Comparison A scientist studies the production of a key digestive enzyme in silk moths. The moths have one gene for this enzyme, and the scientist extracts mRNA transcribed from this gene as well as protein translated from it. The gene has three introns in its sequence. Use the passage to answer the question. Part A: If the researcher compares mRNA from inside the nucleus to mRNA from the ribosomes, what will be found? A. less mRNA in the nucleus B. shorter mRNA in the ribosomes C. identical mRNAs in both places D. mRNA covalently attached to protein in the nucleus Part B: Use the passage to answer the question. If the same scientist extracts the DNA for this gene and finds that it becomes methylated over the life of the organism, how will digestion change over time? A. It will occur at lower pH. B. It will rely more on this enzyme over time. C. It will use methylated copies of the enzyme. D. It will involve a lesser quantity of this enzyme over time.arrow_forwardWhat is the correct order of information flow in bacterial and eukaryotic cells? (a) DNA →mRNA →protein (b) protein → mRNA → DNA (c) DNA →protein→mRNA (d) protein →DNA → mRNA (e) mRNA → protein → DNAarrow_forward
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- Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning