Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Textbook Question
Chapter 20, Problem 1TYU
In DNA technology, the term vector can refer to
- (A) the enzyme that cuts DNA into restriction fragments.
- (B) the sticky end of a DNA fragment.
- (C) a SNP marker.
- (D) a plasmid used to transfer DNA into a living cell.
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Why is DNA ligase so important in recombinant DNA technology?
a.) It causes DNA to make multiple copies of itself.
b.) It joins two DNA fragments together.
c.) It shapes bacterial DNA into a circular plasmid.
d.) It cuts DNA into restriction fragments.
In Biotechnology, gene cloning is a very important technique. A vector is normally required
to perform this process. The vector commonly used to transform a bacterial host cell is the
plasmid.
(i)
State the THREE (3) important regions of the plasmid. Elaborate your answer.
(ii)
Besides plasmids, name TWO (2) other commonly used vectors in Biotechnology.
In Biotechnology, gene cloning is a very important technique. A vector is normally required
to perform this process. The vector commonly used to transform a bacterial host cell is the
plasmid.
(i)
State the THREE (3) important regions of the plasmid. Elaborate your answer.
Chapter 20 Solutions
Campbell Biology
Ch. 20.1 - Prob. 1CCCh. 20.1 - DRAW IT One Strand of a DNA molecule has the...Ch. 20.1 - What are some potential difficulties in using...Ch. 20.1 - VISUAL SKILLS Compare Figure 20.7 with Figure...Ch. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 8TYUCh. 20 - MAKE CONNECTIONS Looking at Figure 20.15, what...Ch. 20 - DRAW IT You are cloning an aardvark gene, using a...Ch. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - Prob. 14TYU
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- Isolation of DNA is a crucial step for genetic engineering. (i) What is the difference between genomic DNA and plasmid DNA? (ii) Describe the common procedure for isolating genomic DNA without using the DNA extraction kit. ..1..arrow_forwardHow are "sticky ends" generated in DNA? A) by gel electrophoresis B) by cutting with restriction enzymes C) by using DNA polymerase D) by adding DNA ligase E) both A and B are correctarrow_forwardA double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition sites. How many fragments will be produced? a) 2 b) 3 c) 4 d) 5arrow_forward
- Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) useHindIII to cut both the plasmid and the human DNA (d) a or b (e) b or carrow_forwardThe polymerase chain reaction uses Taq polymerase rather than a DNA polymerase from E. coli, because Taq polymerasea) introduces fewer errors during DNA synthesis.b) is heat-stable.c) can initiate DNA synthesis at a wider variety of sequences.d) can denature a double-stranded DNA template.e) is easier to obtain.arrow_forwardGENETICS if/when a "whole-genome shotgun" approach is used for DNA sequencing, which of the following is MOST likely to create problems during the assembly of a complete genomic sequence? a) long sequence reads b) a high degree of coverage/ redundancy in the sequence data c) the presence of repetitive DNA d) not enough contigs e) all of the abovearrow_forward
- From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forwardThe transposase enzyme is essential for transposition because A) It can recognize the end of the DNA transposable elements B) It can cut the DNA strands C) It can copy the transposable elements into RNA D) A and B E) A, B and Carrow_forwardWhat occurs during the HIGH temperature step of PCR? A) DNA is cut by restriction endonucleases B) primers get degraded C) primers anneal to single-stranded DNA D) DNA is synthesized E) double stranded DNA separates into single stranded DNAarrow_forward
- You discover an E. coli mutant that has a non-functional DNA polymerase III. Such a bactèria; a) Would not be able to synthesize new strands of DNA b) Would not be able synthesize the leading strand of DNA Oc) Would produce new strands of DNA still containing short RNA primers Od) Would be able to synthesize the lagging strand of DNA onlyarrow_forwardA DNA polymerase enzyme inherently incorporates an incorrect nucleotide at a low but measurable rate. Such a mutation is termed _______. A) spontaneous B) lysogenic C) transformative D) transductive E) inducedarrow_forwardA DNA library is aa) A DNA fragment inserted into a vector.b) A general collection of all genes sequenced thus far.c) All DNA fragments identified with a probe.d) A collection of DNA fragments that make up the entire genome of a particular organism.arrow_forward
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