construct! Which of the four constructs (Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.)

construct! Which of the four constructs (Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.)

Name: construct! Which of the four constructs(Construct 1-4) below could yo
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Description: construct! Which of the four constructs(Construct 1-4) below could you use to makeyour own mouse knockout? For each oneyou did not choose, explain why you did notchoose that construct. Part of DHCR7 genestructure is shown on top row in theschematic.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Mutant # Deletion Region %Transcription (lungs) %Transcription(kidneys) WT None 100% 100% 1 1-200 130% 130% 2 250-400 100% 100% 3 500-800 50% 50% 4 950-1100 0% 100% 1) For the following gene, you notice the following results when analyzing several muntants. What type of sequence (be specific) has been mutated in mutant 1? What type of sequence (be specific) has been mutated in mutant 3? What would we call this type of mutation? What type of sequence (be specific) has been mutated in mutant 4?
Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed in cultured normal human somatic cells (A–G; “-” = control: no expression of exogenous gene/siRNA). The cells were either untreated (-) or irradiated to induce DNA damage (+). After 24 hours, the cells were harvested for preparing cell-free extracts. Different proteins were then detected using SDS-PAGE followed by Western blotting. These include p53, the phosphorylated form of pRb as well as total pRb, the phosphorylated form of lamin B as well as total lamin B, and caspase 3 (note that the darkness of the bands roughly corresponds to the intensity of the bands in the Western blot). Fig.1. The cells were also analysed using flow cytometry. The x-axis is DNA contents (2N represents the position of DNA as in G1 phase; 4N represents the position of DNA as in G2/M phase); the y-axis is the accumulated cell number. Fig.2. (1) The first four samples are known (the rest, see below, are all…
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Describe the primary procedure(key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open usingEcoR1which has a 5¢-recognition site and a 3¢- Hind III in the MCS?
Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?
Which of the four constructs (Construct 1-4) could be made to make a knockout mouse? For each one not chosen explain why you did not choose that construct. Antibiotic selection and negative selections are abbreviated)
amount of protein / amount of mRNA 12 mm / ↑ A B time (minutes) 20 Bacteria were grown in culture. At various time points, substances were added or removed from the culture medium. To assay the effects of the substances on operon gene expression, samples of bacteria were collected at one-minute intervals and levels of specific proteins and mRNAs were determined. If the operon under investigation is a repressible operon, which arrow indicates the addition of a molecule that binds the repressor protein?
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