Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 17, Problem 32P
Interpretation Introduction

Interpretation:

The reason for the GTP molecule or another nucleoside triphosphate is actively similar to an ATP molecule in the metabolism should be determined.

Concept introduction:

The citric acid cycle (TCA) is a catabolic process. It is a series of chemical reactions in which the stored energy of the body is released by the oxidation of acetyl-Coenzyme A. Anaplerotic reaction are those chemical reactions which form the intermediates of a metabolic pathway.

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Chart is Given for you: Below is a chart of values for actual enzymes. Enzyme                Km (M)                       kcat (1/s)Chymotrypsin       1.5 × 10^−2                   0.14Pepsin                   3.0 × 10^−4                   0.5Tyrosyl-tRNA synthetase 9.0 × 10^−4        7.6Ribonuclease        7.9 × 10^−3               7.9 × 10^2Carbonic anhydrase 2.6 × 10^−2             4.0 × 10^5Fumarase               5.0 × 10^−6                  8.0 × 10^2 Assume the enzyme concentration is equal across all samples (and is equal to 1). (Answer a and b only)a. Which enzyme will have the highest V0 at very high substrate concentrations? (1 M). Why? b. Which will have the highest V0 at very low substrate concentrations (5.0 × 10^−12). Why?
Fill in the blanks (write answers only in correct sequence). When the activation energy required is less between the reactants and the products, reaction is known as----- i. ii. The region of an enzyme where substrate molecules bind and undergo a chemical 11. reaction is known as--- 11. To produce amino acids and synthesis of NADH, cycle used is-- Metabolic cycle that takes place both in cytoplasm and mitochondria is--- Glucose 1 phosphate is converted to Glucose 6 Phosphate by the action of - Which vitamin deficiency is linked with the night blindness? Name it iv. V. vi.
Protein synthesis is energetically expensive. Consider the hydrolysis of ATP into AMP as equivalent to 2 ATP, and both ATP and GTP as energetically equivalent. In a cell-free system, how many ATP equivalents are required to incorporate 32 amino acids into a growing peptide chain?
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