Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 7SQ
After a gel electrophoresis procedure is run, the pattern of bars in the gel shows
- a. the order of bases in a particular gene.
- b. the presence of various-sized fragments of DNA.
- c. the order of genes along particular chromosomes.
- d. the exact location of a specific gene in the genome.
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The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does the number of copies of the DNA region being amplified change at the end of each cycle of the polymerase chain reaction?
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Chapter 12 Solutions
Campbell Essential Biology (7th Edition)
Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - Prob. 3SQCh. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - What feature of a DNA fragment causes it to move...Ch. 12 - After a gel electrophoresis procedure is run, the...Ch. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Put the following steps of human gene therapy in...Ch. 12 - For each statement, identify which major theme is...
Ch. 12 - Prob. 11IMTCh. 12 - For each statement, identify which major theme is...Ch. 12 - Some scientists once joked that when the DNA...Ch. 12 - Interpreting Data When comparing genomes from...Ch. 12 - Listed below are 4 of the 13 genome sites used to...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - 18. In October 2002, the government of the African...Ch. 12 - From 1977 to 2000, 12 convicts were executed in...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look?A. The sequence will be readable for the first 20 bases only.B. The detector will detect two nucleotides at about 75% of the positions.C. The sequence will be readable for the last 20 bases only.D. The detector will detect one nucleotide at about 75% of the positions.E. The detector will detect no nucleotides at any of the positions. Explain why it is B.arrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardUse a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the comparative electrophoretic mobilities of DNAs with 150, 600, and 1200 bp.arrow_forward
- (b) Use a drawing to illustrate the principle of DNA gel electrophoresis. +arrow_forwardyou performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level what your gel profile would look like due to this mistake. a. you forgot to add ddNTPs to your test tube. b. you added too much ddNTPs to your test tubearrow_forwarda) Explain the difference between a genome and a transcriptome. Do all cells in an organism have the same genomes and or transcriptomes? b) Explain a method you could use to compare transcriptomes, and what you can learn from comparing transcriptomes.arrow_forward
- With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy nucleotides are fluorescently labeled.b. The dideoxy nucleotides cannot be incorporated into a growingDNA strand.c. When incorporated into a DNA strand, the dideoxynucleotidesprevent further growth of the strand.d. The dideoxy sequencing method is used to determine the basesequence of DNA.e. The dideoxy sequencing method requires the use of primers.arrow_forwardFrom your knowledge about DNA microarray, answer the following: A- How DNA microarray is created? and why it is referred to as “hybridization technology”? B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment? C- Mention the name and the color of the dyes used in expression microarray? D- If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?arrow_forwardYou have two samples you have to sequence: one is a cloned plasmid that you want to verify the sequence of, and another is the cDNA library of the transcriptome of a cell. Which method would you use for each sample and why?arrow_forward
- Give the purpose of gel electrophoresis system. If larger DNA is loaded in the gel, what will be the movement of such DNA, how about is smaller DNA is loaded into the gel?arrow_forwardDuring proofreading, which of the following enzymes reads the DNA? a. primase b. topoisomerase c. DNA pol d. helicasearrow_forwardDescribe the main technique for amplifying a segment of DNA (like the one you suspect is involved in Lee’s cancer) from a complex mixture of genomic DNA. Remember that the entire human genome sequence is known. (Hint: This is a technique that is commonly used by laboratories that do genetic testing and various other applications of molecular biology.)arrow_forward
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