Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 12, Problem 5SQ
Why do DNA fragments containing STR sites from different people tend to migrate to different locations during gel electrophoresis?
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Chapter 12 Solutions
Campbell Essential Biology (7th Edition)
Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - Prob. 3SQCh. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - What feature of a DNA fragment causes it to move...Ch. 12 - After a gel electrophoresis procedure is run, the...Ch. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Put the following steps of human gene therapy in...Ch. 12 - For each statement, identify which major theme is...
Ch. 12 - Prob. 11IMTCh. 12 - For each statement, identify which major theme is...Ch. 12 - Some scientists once joked that when the DNA...Ch. 12 - Interpreting Data When comparing genomes from...Ch. 12 - Listed below are 4 of the 13 genome sites used to...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - 18. In October 2002, the government of the African...Ch. 12 - From 1977 to 2000, 12 convicts were executed in...
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- What is the function of the homogenizing solution in DNA isolation? What are its components?arrow_forwardIt is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forwardWhy do longer DNA molecules move more slowlythan shorter ones during electrophoresis?arrow_forward
- Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move faster than a larger one?arrow_forwardWhat is the cause of an extra band in a DNA ladder in agarose gel electrophoresis?arrow_forwardWhich of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?arrow_forward
- According to the double-strand break model, does gene conversionnecessarily involve DNA mismatch repair? Explain.arrow_forwardIn gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of the electric field? The positive pole? Why are these attractions and repulsions important to the process?arrow_forwardWhat is the mechanism of separation of large DNA fragments in pulsed field gel electrophoresis.arrow_forward
- What is the function of the chaotropic salt in DNA isolation?arrow_forwardThe technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…arrow_forwardWhat is the rationale for the salting out technique for DNA extraction?arrow_forward
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