Campbell Essential Biology (7th Edition)
7th Edition
ISBN: 9780134765037
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 12, Problem 3SQ
Summary Introduction
To determine:
The benefit of using restriction enzyme that cuts the DNA in staggered fashion in making recombinant DNA.
Introduction:
Restriction enzyme are the endonucleases which cuts the double stranded DNA after recognizing specific short sequence of DNA called recognition sequence and cleave the both strand of DNA at or near the recognition site. Restriction enzymes cut the DNA and produce two types of fragments, DNA fragment with blunt end and DNA fragment with cohesive end.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?
What are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?
Restriction endonuclease and ligase are two types
of enzymes used in the process of genetic
engineering, i.e., the manipulation of genes. The
restriction endonuclease differs from ligase in that it
breaks the DNA at ends, while ligase causes
the breaks in DNA from interior
joins the fragments of DNA, while ligase
breaks the DNA into fragments
breaks the DNA at specific points, while the
ligase joins the fragments of DNA
breaks the DNA apart at each nucleotide,
while ligase use the pieces to translate
Chapter 12 Solutions
Campbell Essential Biology (7th Edition)
Ch. 12 - Suppose you wish to create a large batch of the...Ch. 12 - A carrier that moves DNA from one cell to another,...Ch. 12 - Prob. 3SQCh. 12 - A paleontologist has recovered a bit of organic...Ch. 12 - Why do DNA fragments containing STR sites from...Ch. 12 - What feature of a DNA fragment causes it to move...Ch. 12 - After a gel electrophoresis procedure is run, the...Ch. 12 - Name the steps of the whole-genome shotgun method.Ch. 12 - Put the following steps of human gene therapy in...Ch. 12 - For each statement, identify which major theme is...
Ch. 12 - Prob. 11IMTCh. 12 - For each statement, identify which major theme is...Ch. 12 - Some scientists once joked that when the DNA...Ch. 12 - Interpreting Data When comparing genomes from...Ch. 12 - Listed below are 4 of the 13 genome sites used to...Ch. 12 - In the not-too-distant future, gene therapy may be...Ch. 12 - Today, it is fairly easy to make transgenic plants...Ch. 12 - 18. In October 2002, the government of the African...Ch. 12 - From 1977 to 2000, 12 convicts were executed in...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Restriction sites are palindromic; that is, they read the same in the5' to 3' direction on each strand of DNA. What is the advantage ofhaving restriction sites organized this way?arrow_forwardIf you wanted to create recombinant DNA using this enzyme(3' T A 5'), would you have to cut both samples of DNA with this enzyme, or could you use two different restriction enzymes? Explain.arrow_forwardA restriction endonuclease breaks a bacterial plasmid into sticky ends to create recombinant DNA. The same restriction endonuclease is used to cleave the DNA segments that will be added to the plasmid. What are sticky ends, and why are complementary sticky ends on the target DNA and the plasmid it will be inserted into so important?arrow_forward
- What is a restriction digest? What does it mean if you were given a precut DNA?arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forward
- Recombinant DNA construction involves a) Cleaving DNA with a restriction endonuclease and joining with polymerase b) Cleaving and joining DNA with restriction endonuclease c) Cleaving DNA with a restriction endonuclease and joining with ligase Cleaving DNA with ligase and joining with endonuclease d)arrow_forwardWhich restriction enzyme used in your simulated electrophoresis experiment produced DNA with ‘sticky ends’? Which produced blunt ends? Of these two restriction enzymes, which would you choose to use as donor DNA to graft (or splice) onto a recipient strand of DNA, and why?arrow_forwardWhen making recombinant DNA, why must you use the same restriction enzyme to cut the gene of interest and the plasmid?arrow_forward
- You have a recombinant plasmid containing a vector and a segment of foreign DNA, both equal sizes. Draw a picture of this recombinant plasmid labeling foreign and vector regions. Where the foreign DNA meets the vector, there is a cut site for restriction enzyme ABC1. When the recombinant plasmid is cut by ABC1, how many fragments do you expect to be produced? Identify these fragments.arrow_forwardWhich of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be knownarrow_forwardWhat is a restriction enzyme? How can restriction enzymes be used to splice a piece of human DNA into a plasmid?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage Learning
Human Biology (MindTap Course List)
Biology
ISBN:9781305112100
Author:Cecie Starr, Beverly McMillan
Publisher:Cengage Learning
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY