Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 1, Problem 9RQ
Summary Introduction
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The development of recombinant DNA (deoxyribonucleic acid) technology using natural bacterial genetics.
Introduction:
Biochemical and microscopic advances showed the basic structure and function of the cell membranes and proteins. The discovery of DNA and RNA (ribonucleic acid) structures led to the discovery of model bacterial genetic systems, such as E.coli (Escherichia coli)and lambda phage. In addition to
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Briefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at your own words.
Describe the principles behind recombinant DNA technology.
Explain the steps in recombinant DNA technology or rDNA technology
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Microbiology: An Evolving Science (Fourth Edition)
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- Explain the steps of recombinant DNA technologyarrow_forwardJackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forwardDiscuss the relationship between the development of technology, the generation of new theories and the advancement of science-specifically- microbiology. You should a use an historical perspective from van Leewoenhoek to CRISPR. Include new theories, methods, protocols and procedures.arrow_forward
- Describe the basic principles behind recombinant DNA technology.arrow_forwardExplain the purpose of the antibiotic resistance gene in this experiment. Why is this genetic trait an important part of the recombinant DNA technology process in the biotechnology industry?arrow_forwardDescribe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular cloning and genetic engineering.arrow_forward
- Explain the Applications of recombinant DNA technology in medicine ?arrow_forwardAnswer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger printing. n) What is gene cloning? o) Elaborately…arrow_forwardWhat is recombinant DNA technology ?arrow_forward
- Propose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.arrow_forwardExplain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardThe first publications describing the successful production and intracellular replication of recombinant DNA appeared in the early 1970’s. Since then, there is a long list of breakthroughs achieved by recombinant DNA technology. But which one of the following are NOT on that list? Select more than one answer ifneeded. a) Recombinant Factor VIII protein for the treatment of hemophiliacs b) Positional cloning of over 100 human disease genes c) Bacteria producing human insulin for the treatment of diabetic patients d) Reproductive cloning of over a dozen humansarrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license