Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.

Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.3: Culture Techniques For Bacteriology

Problem 5RQ

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An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is transferred to the wire loop but before transfer to new media B. before culture is transferred to the wire loop and after culture is transferred from the wire loop to new media C. after culture is transferred to the wire loop and before culture is transferred from the wire loop to new media D. before culture is transferred to the wire loop

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	A.	Stab the butt of the media with the wire loop.
	B.	Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop.
	C.	Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant.
	D.	Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.