Why do you think the continuous culture has higher chances of contamination than batch and fed-batch culture?

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…

A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…

Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…

A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…

Q: In a bacterial culture on an agar plate, what is a zone of inhibition?     An area where the…

A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: You have a culture that has 5 X103 cfu/ml of bacteria in it. You perform a serial dilution by adding…

A: In microbiology and bacteriology, the technique of serial dilution is a fundamental method used to…

Q: You found a bright blue growth on a tomato you had left on your kitchen counter. You’ve never seen a…

A: Introduction  A hypothesis is a proposed explanation for a phenomenon or observation that can be…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: A bioreactor which is used for the production of growth factor A is operated as fed- batch culture.…

A: A Bioreactor is a device or a process system which supports and habitats the biologically active…

Q: Why is it necessary to make pure subcultures of organisms grown from clinical specimens? How does…

A: Introduction: A subculture is developed by transferring several cells from an earlier culture to a…

Q: Which cultures would be suitable for the transduction experiment and why？ Which cultures would not…

A: Transduction is a process of gene transfer in bacteria, where bacterial DNA is transferred from one…

Q: he image below is the results of the Nutrient agar plate inoculated with the kitchen ounter sample…

A: Colony morphology is used by microbiologists to identify and characterized the different bacterial…

Q: What is the difference between the pour plate method and the spread plate method? Why might you…

A: Standard plate count method is a growth based approach that counts the number of living…

Q: An attempt to transfer bacteria into new media during the death (Decline) phase of a culture…

A: Microbial cultures, also known as microbiological cultures, are made by allowing microbial organisms…

Q: How reliable is PCR to detect microorganisms in water samples?

A: Polymerase chain reaction (PCR) is a technique discovered by Kary Mullis, which replicates DNA…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…

A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…

Q: The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells.…

A: The passage number of a cell culture is a record of the number of times the culture has been…

Q: Can culture media be placed in a freezer? Why or why not

A: Culture media are also called as growth media. They are mixture of nutrients and other substances…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Why after a certain period the growth rate of the culture decreases until growth stops?

A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…

Q: What should be taken into consideration in the selection of the culture preservation method?

A: The problem of maintaining microbial culture is one that many fields of microbiology face. The…

Q: A broth culture is initially inoculated with a single bacterial cell. For the first hour the…

A: * Given that  The bacteria has generation time of 30 minutes. So it takes about thirty minutes to…

Q: Below is a set of data for a serial dilution/plate count experiment.    Based on this data what is…

A: There are numerous methods/techniques for counting the number of bacteria in a particular sample. A…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: What is the confluency of the culture below? O 15% 80% - 90% 75%

A: In cell culture biology, confluence refers to the percentage of the surface of a culture dish that…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: temperature and light source affect the number and type of colonies that can be found in the…

A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…

Q: You are hired in one of the microbiology labs in Memphis and your first assignment to identify…

A: 9. Gram-negative bacteria are characterized by their cell walls, which have a thinner layer of…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: Is it likely that a pure culture could be isolated from the human body? Why or why not?

A: In microbiology the pure culture is a laboratory culture that contains a single species of organism.…

Q: If you counted 300 colonies on a plate that had a 10-5 dilution of bacteria culture, how many…

A: Introduction  Colonies are groups of microorganisms, such as bacteria or yeast, growing on or within…

Q: differential plate enrichment plate selective plate streak plate pour plate

A: Differential plate:  Differential media used to distinguish between two related microbes. It used to…

Q: Given the scenario, compute for the total volume of the culture media solution (milliliter or liter)…

A: We have been given the concentration of the nutrient broth and Agar and we have also been given the…

Q: Why should bacterial culture plates be incubated inverted (bottom up)?

A: Microbial isolation is a process done in microbiology lab in order to obtained microbe of interest…

Q: Why does dry heat require higher temperatures for longer time periods to sterilize than moist heat

A: Sterilization may be defined as the process which help in the complete removal of all…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…

Q: What is the purpose of a bacterial smear? What is the purpose of a bacterial smear? It spreads the…

A: Introduction:  The bacterial smear technique is a routine procedure performed to prepare the…

Q: ow can one be sure that the culture media are sterile or free from contamination

A: Sterility, often known as sterility, is the state of being free of all contaminants and…

Q: You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture…

A: The objective of this question is to understand how to make a 10^-2 dilution of a bacterial culture…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.