You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 4.0 mL to the 99 mL. A.) What was your intended dilution factor?  B.) What was your actual dilution factor?

You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies. What was the original concentration in the sample? Show your calculations.

What is moisture content of test results.

The stool samples submitted by the two friends were enumerated following enrichment. The technician prepared serial dilutions. A volume of 1 mL of stool was mixed with 1 mL of diluent. From this dilution, they then took 1 mL and added it into 1 mL of diluent. The operation was repeated 5 more times. After finishing the serial dilution, 100 μ�L has been spread on different TSA plates. Following 24h incubation, the technician counted 23 colonies at the dilution 4.   What is the bacterial concentration in the enriched culture? [1 mark]

To measure the culture temperature, why is the thermometer placed in a tube separate from the culture?

how to find out the test is differential or selective in the microbiology lab test?

When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?

Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml blanks. Show how you would plate out 102 through to 10-6

You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?

The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?