困Topic: laboratory InstrumentWhich of the following are TRUE regardingWhich of the following is/are TRUEthe use of laboratory instruments andregarding the use laboratory instrumentsequipment in regards to making of culturemedia? "and equipment? *Air bubbles on the surface of agar platesHot plates can be used to sterilize theinoculating loop and needle.can be removed by fanning them with theluminous flame of the bunsen burner.If the agar surface is wet, it can be dried byA drying oven is used to sterilize nutrientagar, a solid culture medium.heating at 30-40 deg C in the drying ovenbefore inoculation is done.The refrigerator is used to growpsychrophiles.All media taken from the refrigerator shouldbe warmed to room temperature beforeuse,P Type here to search7:11 pm30/09/2021ヘロG) ENG近

INTRODUCTION The answers on the topic laboratory instruments are given below.

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Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter18: Instrumentation In The Operating Room

Section: Chapter Questions

Problem 3CS

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A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which characteristic of the species used determines whether sterility was achieved when autoclaved?
A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?
A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?
What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
Determine what percentage of the culture was living (viable) and what percentage was dead (mortality). Plates Plate Dilution Volume plated No.of colonies Avg No Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 1 10^-3 10ul R1=130,R2= 110,R3=210 150 150mL 1.50*10^6 Volume of cells(mL) Volume of diluent(mL) Total dilution(D) Hemocytometer count Avg cells in 1 mm^2 area Concentration of diluted sample Cd(cells/mL) Concentration of original sample Cu(cells/mL) 4.3 0.5 0.1 grid 1= 171 , grid2 = 185 178 1.78*10^5 1.78*10^6
In product testing, microbial growth is found.| Interpretation: Rationale:
A swab for culture is received from a doctor's office. The staff forgot to mention thesource from where the swab was taken and the climc was closed for next few days.Whatdifferent selective/differential media may be helpful to you to identify the organism.Explain very briefly your rationale for using the particular culture media.
What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
Mention any three (3) fluids used in cleaning lenses in the microbiological laboratory.
Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled % Absorbance 0 min 5% 30min 7% 60min 9% 90min 15% 120min 25% 150min 37% 180min 49% 210min 50% 240min 49% 270min…
indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…
I need help with microbiology What is the magnification of each of the following objectives? scanning: low power: high dry: oil immersion:

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