In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agarplate?in the agar directly below the discO in the agar halfway between the disc and the petri dish wallO in the agar furthest away from the discthe cehmical is equally distributed throughout the agar

The disc diffusion method was developed by Kirby and Bauer to determine the efficiency of a particular drug. One can use this protocol for estimating the antimicrobial activity of a particular substance. The protocol includes impregnation of a disk (drug disk) in the culture plate which is cultured by a specific bacterial strain for which one wants to check antimicrobial activity. The drug slowly and slowly diffuses in the agar plate and the region where its concentration is maximum results in the development of a zone of inhibition (if the drug has an antimicrobial effect) which means that the drug is antimicrobial. The diameter of the zone of inhibition is estimated for further analysis The correct statement is C When the drug is impregnated into the culture plate, it slowly diffuses out from its region of high concentration to low concentration, but its lowest concentration would be the region where agar is farthest from the disk as diffusion is the slow process and soon zone of inhibition would be formed which is the utility of protocol rather than the assurance that it has reached to all regions of culture plate or not.