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- What are the possible problems/limitations to be encountered in a laboratory experiment about the Isolation of Foodborne Pathogens on Selective, Differential, and Enriched media (e.g., blood agar, mcconkey, mannitol, XLT-4) using steak plating method. Provide a brief but concise explanation for every limitations, respectively.How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
- Why is the looping out method preferable for sputum specimen for acid fast smears?The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
- What is the purpose of flame sterilizing the inoculating loop or needle before and after usingit? What is meant by ‘streak dilution’ technique? What is the purpose of placing the inoculated agar plate in an inverted position in theincubator?How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate?Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
- I am wondering whether E. coli can be used as spiked in sample for Bacteria vaginosis detection through qPCR? Please help me with the information including how to purchase it.You were given a mixed nutrient agar broth culture of bacteria 1a. How will you determine the different types of bacteria present in the mixed culture without using an agar plate 1b. How will you make a pure culture of these bacteria on a slant nutrient agar 1c. How will you identify these bacteria from the pure culture without using an agar plateBased on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *
