Q: List and explain the Sterile Technique What's the difference between Medical vs. Surgical Asepsis?…
A: Aseptic technique or Sterile technique means using practices and procedures to prevent contamination…
Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…
A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…
Q: explain how the following genotypic and phenotypic methods of microbe identification is done. Use…
A: The biochemical tests are the tests that are used to identify various types of microbial species.…
Q: For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box…
A: Staphylococcus aureus is a spherical-shaped cocci that belongs to the family of Firmicutes. It is…
Q: explain why it is not good to break sterile field when inserting a foley catheter
A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…
Q: Why is mannitol salt agar (MSA) considered both a selective and differential agar for staphylococci?
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: In what instances would the pour plate method be more appropriate than the spread plat method?
A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…
Q: Differentiate the following artificial media used in animal cell culture: serum-containing media…
A:
Q: Considering microbial identification methods, to which category would the following method belong…
A: Microbial identification plays very crusial role in studying the growth of microorganism, with…
Q: n a culture tube labeled X as your unknown. Outline the 7 steps that will enable you to determine…
A: Being able to differentiate microorganism species is very important for a number of reasons, from…
Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…
A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…
Q: When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times…
A: The initial identification of the streptococcal and enterococcal species is based on their hemolytic…
Q: Give the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions…
A: Antibiotic discs are used in microbiological studies to observe the effects of various antibiotics…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: Negative Control Unknown Isolate
A: Blood agar comes as a basal media and can be used as a general growth medium. Blood agar is an…
Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…
A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: You counted 40 colonies on a plate in your dilution series. The plate was inoculated with 1.0ml from…
A: A microbial sample will have enormous amount of cells. To estimate this by calculation, the sample…
Q: what is the diffrence between Aseptic technique and sterile technique
A: Aseptic technique Also called as asepsis. It means absence of germs such as bacteria, viruses, and…
Q: Explain why isolating a pathogen through standard culture methods may become an outdated diagnosis…
A: The term isolation refers to the separation of a strain from a natural, mixed population of living…
Q: Cause and Effect Analysis describe the effect on the test procedure and explain briefly The zones…
A: Determination of bacterial resistance to antimicrobials is an important part of the management of…
Q: On a spread plate, you count 50 colonies. The plate was created from a 10^-2 dilution and 0.2 mL of…
A: Microbial culture refers to the method of letting the micro-organisms multiplying in the culture…
Q: What is the principle behind invertase test
A: Invertase was an enzyme that hydrolyses the disaccharide sucrose into monosaccharides glucose and…
Q: When using the blue/ white colony screening method, what do blue colonies represent ?
A: Blue- white screening is used for the identification of recombinant bacteria.
Q: Is phenol red test a efficient test for unknown intestinal bacteria?
A: Fermentation of carbohydrates is the method used by microorganisms to produce energy. Phenol Red…
Q: What is the color result of tryptophan,tyrosine and phenylalanine in xanthoproteic test? Results and…
A: Xanthoproteic test was a specific test that was done to detect the presence of aromatic amino acids…
Q: Which test require a straight line inoculation? Choose all that apply
A: Answer: STRAIGHT LINE TECHNIQUE : It is the streaking technique of bacterial colony over the agar…
Q: Which of the following would suggest your stock culture is contaminated? Gram stain indicates…
A: A contaminated culture has had contaminants introduced into it. Contaminants are unwanted microbes…
Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…
A: Both are gram positive bacteria.
Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:
A: Bacterial cultivation requires specific types of medium that can provide some of the basic…
Q: erial slide agglutination technique important in diagnostic procedure?
A: Immunoassays are bioanalytical ways that use the specificity of an antigen-antibody reaction to…
Q: Considering microbial identification methods, to which category would the following method belong…
A: There are different types of microbial identification methods, Immunological-Serological analysis,…
Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…
A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…
Q: How do you know if you have a ‘countable’ plate when doing a dilution series? Two plates…
A: The concentration of stock solution can be calculated by first diluting the stock into a suitable…
Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…
A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: Between Bacillus megaterium, Bacillus sphaericus, and Bacillus subtilis which are positive or…
A: The VP test detects organisms that utilize the butylene glycol pathway and produce acetoin. It…
Q: Write a detailed description of the following plate 1- blood Agar plate 2-PEa Agar Plate
A: An agar plate refers to a Petri dish containing an agar solidified growth medium which is meant for…
Q: Describe conventional techniques used to isolate single colonies and establish a pure culture
A: Answer: Introduction: Pure culture a culture contains growth of only single organism without other…
Q: Diagram illustrating Miles & Misra technique for determining viable counts: 10ul 10-5 106 104 10-7…
A: Miles and Misra technique in microbiology helps us to calculate the number of surface viable cells…
Q: Describe the step-by-step procedure for preparing a thick and thin smear to test for malaria.
A: Malaria is a vector-borne disease that is caused by the parasite Plasmodium. This disease is…
Q: Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar…
A: # Here I am giving solution of first two questions . Please repost your third question and better to…
Q: discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple…
A: A mixed culture is one that contains more than one type of organism growing in a sterile medium. The…
Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…
A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…
Q: Describe results from a coagulase, DNase, and novobiocin test that would suggest a mixed culture was…
A: Staphylococci are Gram-positive spherical bacteria that occur in the form of grape-like clusters.…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: DESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.
A: Microbes, which are tiny and nearly invisible, have had a huge influence on society since the…
Q: Why are aseptic techniques implemented during inoculation?
A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…
Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay adam's method).
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- Explain why culture based biochemical and genetic testing would not be the ideal method to identify the microbe.What are the different manufacturing methods of Suppositories dosage form? Explain heat molding process using flow chart. Please explain at your own words.If an organism that showed no growth in the MIC broth test at a particular dose, then grows in an MBC test when plated to media, the concentration of the drug was bacteriostatic. O 1) True O 2) False
- What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?Which of the following is FALSE about interpreting the results of normal microbial flora experiment? 1. Blood plates demonstrate the presence of pathogenic Beta Hemolytic Strep 2. There may be Staph specie, fungus and Strep species present 3. The presence of S. aureus could mean you are carrying MRSA 4.Plates with no growth means the individual is very healthy 5.No growth on Sabouraud agar means you are not carrying any yeast on yourDescribe conventional techniques used to isolate single colonies and establish a pure culture
- Describe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.In what instances would the pour plate method be more appropriate than the spread plat method?What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage Disadvantage
- a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?1. Draw and describe the possible result that may be observed on an agar plate that utilized a 4- streak quadrant method for isolation WITHOUT the conduct of proper aseptic techniques Source of inoculum: Tap water Characteristic of colony: Yellow, circular colony with entire margin and convex elevation Answer: 2. Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. Unwashed Washed Answer. 3. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (C) texture, and (d) optical property.1. Draw and describe the possible result that may be observed on an agar plate that utilized a 4-streak quadrant method for isolation WITHOUT the conduct of proper aseptic techniques Source of inoculum: Tap water Characteristic of colony: Yellow, circular colony with entire margin and convex elevation Answer: 2. Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. Unwashed Washed