1. You were given a mixed nutrient agar broth culture of bacteria.  a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?

Q: There are two bacteria, and I have to find out the organism. I did gram staining, and I found out…

A: Gram staining is a laboratory technique used to differentiate and classify bacteria into two major…

Q: 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original…

A: Dilution is the process of diluting or mixing two or more substances or compounds; it is also known…

Q: What are the 2 most important reasons for using a positive control in Microbiology tests? What are…

A: A control test is used to set a reference for comparison in microbiological tests. It helps the…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: 1. Provided with the following data, compute the corresponding CFU/ml of the original culture.…

A: Various bacterial species are responsible for a variety of diseases in humans. As a result, we apply…

Q: Which one of the following methods of antimicrobial susceptibility testing requires a dilution…

A: Antimicrobial susceptibility testing is a method which is used for determination of antimicrobials…

Q: This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken…

A: A colony-forming unit (CFU) is a unit that is used in microbiology to measure the viable number of…

Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.

A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…

Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…

A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…

Q: While working with Mannitol Salt Agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar (MSA) is a media which has high concentration of salt solution. There are found…

Q: he image below is the results of the Nutrient agar plate inoculated with the kitchen ounter sample…

A: Colony morphology is used by microbiologists to identify and characterized the different bacterial…

Q: The following media are used to grow bacteria for various purposes. Match the examples of media that…

A: MacConkey Agar: - Selective Growth Medium Malt Extract Agar:- Complex Medium

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: What is it called when you stab the nutrient agar during the inoculation step of isolating a…

A: Introduction :  A solid or liquid media that contains nutrients and is used to cultivate bacteria or…

Q: 1) What are all of the different colony types that can be seen on a mixed-culture streak plate?  2)…

A: Hi! Thanks for your question. As you have posted multiple questions, I am answering only the first…

Q: while working with mannitol salt agar you notice the growth of staphylococci however other…

A: Mannitol Salt Agar is used as a selective and differential medium for the identification and…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in…

A: oxidative /fermentative test is used to distingush between aerobic bacteria which oxidize…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: culture that contains multiple types of organisms.

A: The microbiological culture or microbial culture can be defined as the method of multiplication of…

Q: You are hired in one of the microbiology labs in Memphis and your first assignment to identify…

A: The last reagent used in Gram staining, which is crucial for differentiating between Gram-positive…

Q: A broth culture is initially inoculated with a single bacterial cell. For the first hour the…

A: * Given that  The bacteria has generation time of 30 minutes. So it takes about thirty minutes to…

Q: Can you give me an example of a specific medium in culture media and fill the information below.…

A: Selective media are those that prevent the growth of undesirable microorganisms while promoting the…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: Calculate the CFU/mL of the original culture for the countable plate as shown in the diagram. b) How…

A: Introduction :- CFU( colony forming units ) are the count of cells present in orginal culture which…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: To determine if the type of agar affects bacterial growth, a scientist cultures on fou different…

A: Agar is galactose based heterogeneous polysaccharide. Agar is made from red algae. The main…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: How should the urine sample be taken? And why is this technology chosen?  b. A rapid test is…

A: Young women applies to her health centre with burning and frequent vomiting but has no fever.  Acute…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: Which of the following can be used to distinguish one colony from another on an agar plate?  Select…

A: A colony is a visible cluster of microorganisms like fungi and bacteria that grow on a solid growth…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: One ml of a sample was added to 99 ml of buffer. 100 µ of this was plated in nutrient agar. After…

A: A colony-forming bacterium is a microorganism that is capable of growing and dividing on a solid…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…

A: Microbiological research necessitates the use of pure cultures. Spreading bacteria on the surface of…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.