1. You were given a mixed nutrient agar broth culture of bacteria.  a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?

Among the many methods, please identify Bacillus cereus bacteria, in staining reactiontests, such as A.simple staining B.gram staining C. isolation tests To distinguish specific cultural characteristics of Bacillus cereus bacteria, please explain how to identify Bacillus cereus in these tests below? D indole test, E.starchhydrolysis test F. citrate test.

Based only on your EMB and MSA results, build a dichotomous key that could differentiate between these four strains.

You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volume

You are working through the Gram Staining procedure on a mixed culture of S. aureus (G+) and E. coli (G-) (gram + & gram -) organisms respectively. If you accidentally use too much alcohol / acetone during the procedure, which of the following will likely happen? E. coliE. coli will appear to be Gram + will appear to be Gram +E. coli will appear to be Gram + S. aureus will appear to be Gram - Both organisms will appear colorless Both organisms will appear purple None of the answers are correct

you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)

4) You are interested in the total bacterial load of the bat guano. In order to determine this, you go back to your original broth culture and prepare a dilution series. You then spread 100 ml of each dilution onto 3 separate plates. You obtain the following results:   Plate 1, 10 -1 dilution: 362 colonies Plate 2, 10 -3 dilution:  76 colonies Plate 3, 10 -5 dilution:   8 colonies.   Based on your results, calculate the CFUs/ml in your original broth culture. show your work

you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.

What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable number

Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count?  A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply.  ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms?  A. inoculum B. culture medium C. diluent D. microbial culture