Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture
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Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply.
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- Define the following terms:a.Simple stainingb.Complex mediumc.Defined (synthetic) mediumd.Parfocale.Negative stainingf. Resolving powerg.Magnifying powerWhile conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before staining
- Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingWhich of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells
- A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative StainWhat is the Description and uses of these type of microsope?Explain in 2-3 sentencesDescription:Uses: a. Bright Fieldb. Phase Contrastc. Fluorescenced. Confocale. Transmission EMf. Scanning EMWhich of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.
- While conducting the Hydrogen Sulfide Test you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Explain how the color change is to occur. Think critically.A mixed culture is known to contain Gram negative bacilli and Gram positive cocci. You perform a Gram stain of this mixed culture as a control while simultaneously Gram stainin an unknown organism on the same slide (the same procedure you used in lab). The results, observed under the 100x objective lens, are described below: Results: Control side: purple bacilli and purple cocci Unknown side: purple bacilli Using all the information given above, discuss the results of your unknown organism. Shoul you be confident in this result? Answer question A if you are confident, answer question B if you are not confident. a. If you are confident in your results, why? Explain how the staining reagents produced the described results. b. If you are not confident, do you think an error was made in the Gram staining procedure? If so, describe the error and explain how it impacted your results.In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.