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What are the advantages and disadvantage of using spectrophotometry and CFU counting to estimate bacterial growth
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- What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.Collecting data that can then be transformed into a bacterial growth curve is usually done using a spectrophotometer and using optical density. Research and briefly describe how this experiment is completed. What are some of the advantages of using optical density over something like plate count methods?What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank you
- What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlIdentify one culture-based and one non-culture-based method used for analyzing bacterial growth.
- What are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES(1) What is the difference between specific growth rate and generation time? (2) Why is there a need to adjust the optical density of a pre-culture against a McFarland standard or measure it using a spectrophotometer?Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.