What are the advantages and disadvantages of using the Wet Mount technique?

- What is a pure culture? - Describe the procedure for making a streak plate from a clinical specimen (like a urine culture). - What is the desired outcome (result) of a streak plate? - Clinically, why is it important to make a pure culture from a clinical specimen? What do you do next in order to treat the patient?

Create a graph of this spectrophotometric data (using Excel or the graph paper below), labeling the four stages of the growth curve- lag, log (exponential), stationary, death. Be sure to label your axes properly and give your graph a title Time Culture Sampled                        % Absorbance 0 min                                                       5% 30min                                                              7% 60min                                                              9% 90min                                                              15% 120min                                                            25% 150min                                                            37% 180min                                                            49% 210min                                                            50% 240min                                                            49% 270min…

Why is it important to limit the quantity of cells used to prepare a smear?  Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results  2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observed