Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the following enzymes can generate compatible ends for enzyme 1. The recognition site for enzyme 1 is 5'-G^GTACC-3' (^ is the cleavage site). The recognition sites for available enzymes are given as the choices. Select one: a. GGTAC^C b. CGTAC^G c. CACTAGG d. C^GATCG e. C^GTACG ✔ f. GGT^ACC g. CGT^ACG

A. Diagram a short single strand of DNA 5’ -AA-GG- 3’. Show the chemical structure of the phosphoribosyl backbone and the attachment point for nucleotides added as “A” or “G”. B. Diagram the product of digestion was a restriction enzyme to cut this sequence between the A and G.

In Northern blot analysis, mRNA samples from tissues are bound to a labeled DNA probe that is complementary to the mRNA, and run on a gel to be visualized. The protein tropomyosin is known to be present in both brain and liver.  When brain and liver tissue were assayed for the presence of tropomyosin mature mRNA, bands of two different sizes were seen. Tropomyosin gene diagram (3000 bp total): Shown in attatched image If the band on the Northern blot for mRNA isolated from liver tissue is 2580 bp, whereas from brain tissue the band is 2250 bp, what is most likely? a)The two mRNAs are made from different tropomyosin DNA sequences. b)Exon 2 is alternatively spliced out of the brain mRNA. c)Introns 1 and 2 are spliced out of the brain transcript but not the liver transcript. d)Exons 1 and 3 are spliced out of the brain transcript but not the liver transcript. e)Exon 2 is alternatively spliced out of the liver mRNA.

The sequence below shows 140bp of a double-stranded DNA sequence coding for histone H2A. What two primers could be used to amplify the whole 140bp sequence in a PCR reaction? 1                                                                   70ATGTCTGGACGTGGCAAGCAGGGAGGCAAAGCCCGCGCTAAGGCCAAGACTCGCTCTTCTAGGGCCGGTCTACAGACCTGCACCGTTCGTCCCTCCGTTTCGGGCGCGATTCCGGTTCTGAGCGAGAAGATCCCGGCCAG 71                                                                 140 TCCAGTTCCCCGTGGGCCGAGTGCACCGCCTGCTCCGCAAAGGCAACTATGCCGAGCGGGTCGGGGCCGGAGGTCAAGGGGCACCCGGCTCACGTGGCGGACGAGGCGTTTCCGTTGATACGGCTCGCCCAGCCCCGGCC   a. TACAGACCTGCACCGTTC and GGCCGGGGCTGGGCGAGC   b. ATGTCTGGACGTGGCAAG and CCGGCCCCGACCCGCTCG   c. ATGTCTGGACGTGGCAAG and GCTCGCCCAGCCCCGGCC   d. TACAGACCTGCACCGTTC and CGAGCGGGTCGGGGCCGG

Will restriction sites for an enzyme that has 4 bp in its recognition sequence be closer together, farther apart, or similarly spaced, on average, compared with those of an enzyme that has 6 bp in its recognition sequence? Expalin your reasoning?

You have two mutants of pQE. 1-CRYGD. One is 66 ng/uL and the other is 337 ng/uL. You need to add 1 ng to your competent  NEB Express Iq cells.  A. Can you accurately pipet the plasmid for each? Why or why not? B. How could you dilute the plasmid to obtain 50 uL of 0.5 ng/uL? C.How much of the new 0.5 ng/uL sample would you need to pipette for 1 ng

Cancer cells must be able to proliferate indefinitely. Therefore, many inappropriately express telomerase. a. What is the function of telomerase? b. Why does telomerase enable cells to proliferate indefinitely?

Utilizing Hind III and EcoR V Restriction Enzyme with Pet41 and the following gene of interest... a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781…

Tripartite motif-containing protein 5a or TRIM5a is a cellular antiviral restriction factor. Briefly describe the mechanism that prevents early replication events