Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?
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Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?
Turbidity measurement is one of the methods to measure the microbial cultures, helps determine the cell number in culture.
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- Mannitol salt agar (MSA) only allows the growth of halophiles. Among the halophiles, mannitol fermenters release acid that turns the pH indicator yellow; mannitol non-fermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol non-fermenter and a non-halophilic mannitol non-fermenter. What are your expected results in this experiment? Explain the role of the media in this case.explain the various factors affecting agar-agar gels and what is the source, manufacturing, and properties of agar-agar and what is the uses of agar-agar in food products?A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.
- 4. (a) Which of the following chemical reactions is an overall oxidation-reduction (redox) reaction that involves oxidation number changes in their reactions? A. NaOH + H₂O → Na¹ + H3O+ + OH B. HCl + H₂O → H3O+ + Cl- C. CO₂ + H₂O → H+ + CO3²- D. N₂ + 3H₂ → 2NH3How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlCollecting data that can then be transformed into a bacterial growth curve is usually done using a spectrophotometer and using optical density. Research and briefly describe how this experiment is completed. What are some of the advantages of using optical density over something like plate count methods?
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- Describe how to most easily calculate the generation time for a population of bacteria in a batch culture in a closed system. Also explain why the calculation is made in a special phase of the growth curveConsider the desired phenotype pattern of candidates that we wish to advance into experiments in Week 5 and beyond. If the growth patterns illustrated below are observed in your Week 4 results, which of the candidates would you select for further analysis? Select all that apply. Candidate A B A B C с = vigorous growth on this medium = impaired/slow growth on this medium = no growth on this medium SC-H B SC-K A B YP GalName 2 indirect methods for measuring microbial growth and describe how each is performed. a. i. Advantage: ii. Disadvantage: b. i. Advantage: ii. Disadvantage: