Question #2: Consider the following data for Cell G run in 1 L chemostat, with a feed containing 30 g/L substrate: Feedrate (ml/hr) (c) Calculate 27.5 24.2 22.1 18.8 Steady State Cell Mass Concentration (g/L) 12.0 14.7 15.8 16.7 Steady State Substrate Concentration (g/L) 10.0 5.56 (d) Using the calculated kinetic parameters, 3.70 (a) Determine the kinetic parameters for growth (b) At each dilution rate, calculate Yx/s. Is it constant with dilution rate and substrate concentration? 2.23 (i) the critical dilution rate, D. (ii) the dilution rate corresponding to maximum productivity, Dm (iii) the maximum cell productivity (cells/L/hr) (i) Generate a plot of X and S vs D for a feed concentration of 25 g/L. (ii) What is the critical dilution rate for this condition? (iii) What is the dilution rate corresponding to maximum productivity at this condition? (iv) What is the maximum cell productivity at this condition? (v) For a reactor operating at this maximum productivity, what would be the volume required to produce 1000 kg cells/yr? The reactor would operate 300 days/year, 24 hr/day.
Question #2: Consider the following data for Cell G run in 1 L chemostat, with a feed containing 30 g/L substrate: Feedrate (ml/hr) (c) Calculate 27.5 24.2 22.1 18.8 Steady State Cell Mass Concentration (g/L) 12.0 14.7 15.8 16.7 Steady State Substrate Concentration (g/L) 10.0 5.56 (d) Using the calculated kinetic parameters, 3.70 (a) Determine the kinetic parameters for growth (b) At each dilution rate, calculate Yx/s. Is it constant with dilution rate and substrate concentration? 2.23 (i) the critical dilution rate, D. (ii) the dilution rate corresponding to maximum productivity, Dm (iii) the maximum cell productivity (cells/L/hr) (i) Generate a plot of X and S vs D for a feed concentration of 25 g/L. (ii) What is the critical dilution rate for this condition? (iii) What is the dilution rate corresponding to maximum productivity at this condition? (iv) What is the maximum cell productivity at this condition? (v) For a reactor operating at this maximum productivity, what would be the volume required to produce 1000 kg cells/yr? The reactor would operate 300 days/year, 24 hr/day.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Question #2: Consider the following data for Cell G run in 1 L chemostat, with a feed containing
30 g/L substrate:
Feedrate
(ml/hr)
(c) Calculate
27.5
24.2
22.1
18.8
Steady State Cell
Mass Concentration
(g/L)
12.0
14.7
15.8
16.7
Steady State Substrate
Concentration
(g/L)
10.0
5.56
(d) Using the calculated kinetic parameters,
3.70
(a) Determine the kinetic parameters for growth
(b) At each dilution rate, calculate Yx/s. Is it constant with dilution rate and substrate
concentration?
2.23
(i) the critical dilution rate, D.
(ii) the dilution rate corresponding to maximum productivity, Dm
(iii) the maximum cell productivity (cells/L/hr)
(i) Generate a plot of X and S vs D for a feed concentration of 25 g/L.
(ii) What is the critical dilution rate for this condition?
(iii) What is the dilution rate corresponding to maximum productivity at this condition?
(iv) What is the maximum cell productivity at this condition?
(v) For a reactor operating at this maximum productivity, what would be the volume
required to produce 1000 kg cells/yr? The reactor would operate 300 days/year,
24 hr/day.
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